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Role of Lipopolysaccharide and Nonspecific Porins of Yersinia pseudotuberculosis in the Reception of Pseudotuberculous Diagnostic Bacteriophage

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Abstract

The role of the outer membrane components of Yersinia pseudotuberculosis and Yersinia pestis in the adsorption of pseudotuberculous diagnostic bacteriophage (PDB) was assessed with two model bacteriophage-antigen systems, in which the target antigens were either in the solution or on the surface of polystyrene microspheres. It was established that the nonspecific OmpF and OmpC porins of Y. pseudotuberculosis, along with lipopolysaccharide, are involved in the reception of PDB. It was determined that molecular conformation of the porins plays a significant role in interaction with the bacteriophage. It was found that the O antigen of Y. pseudotuberculosis not only does not participate in the reception of PDB but also prevents it by shielding the receptor site on the Yersinia lipopolysaccharide core, which remains completely accessible for the bacteriophage on Y. pestis cells.

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Funding

The work was supported by a Grant of the President of the Russian Federation (grant no. MK-3383.2021.1.4).

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Authors and Affiliations

  1. Vyatka State University, 610000, Kirov, Russia

    L. G. Dudina, I. V. Konyshev & A. A. Byvalov

  2. Institute of Physiology, Komi Research Center, Ural Branch, Russian Academy of Sciences, 167982, Syktyvkar, Russia

    L. G. Dudina, I. V. Konyshev & A. A. Byvalov

  3. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 690022, Vladivostok, Russia

    O. D. Novikova, O. Yu. Portnyagina & V. A. Khomenko

Authors
  1. L. G. Dudina
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  2. O. D. Novikova
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  3. O. Yu. Portnyagina
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  4. V. A. Khomenko
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  5. I. V. Konyshev
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  6. A. A. Byvalov
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Correspondence to O. D. Novikova or A. A. Byvalov.

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Translated by A. Bulaev

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Dudina, L.G., Novikova, O.D., Portnyagina, O.Y. et al. Role of Lipopolysaccharide and Nonspecific Porins of Yersinia pseudotuberculosis in the Reception of Pseudotuberculous Diagnostic Bacteriophage. Appl Biochem Microbiol 57, 426–433 (2021). https://doi.org/10.1134/S0003683821040049

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