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Evaluation of cell wall-associated direct extracellular electron transfer in thermophilic Geobacillus sp.

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Abstract

In this study, a cell wall-associated extracellular electron transfer (EET) was determined in the thermophilic Geobacillus sp. to utilize iron as a terminal electron acceptor. The direct extracellular transfer of its electrons was primarily linked to the cell wall cytochrome-c and diffusible redox mediators like flavins during the anoxic condition. Based on the azo dye decolouration and protein film voltammetry, it was revealed that, in the absence of surface polysaccharide and diffusible mediators, the cell wall-associated EET pathway was likely to be a favorable mechanism in Geobacillus sp. Since the permeability of such redox molecule is primarily limited to the cell wall, the electron transfer occurs by direct contact with cell wall-associated cytochrome and final electron acceptor. Furthermore, transfer of electrons with the help of redox shuttling molecules like riboflavin from cytochrome to cells, vice versa indicates that Geoabcillus sp. has adopted this unique pathway during an anoxic environment for its respiration.

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Acknowledgements

We want to thank Dr. Shivayogeeswar Neelagund, Associate Professor, Department of Biochemistry, Kuvempu University, for his constant support throughout the process. DMG would like to thank all colleagues from the Department of Biotechnology, GM Institute of Technology, Davangere. SIM would like to thank all colleagues from the Department of Biochemistry, School of Applied Science, REVA University, Bangalore.

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Authors and Affiliations

  1. Department of Biotechnology, GM Institute of Technology, Davangere, Karnataka, 577006, India

    Dummi Mahadevan Gurumurthy

  2. School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, China

    Muhammad Bilal

  3. Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, Solan, 173234, Himachal Pradesh, India

    Ashok Kumar Nadda

  4. Department of Biochemistry, School of Applied Sciences, REVA University, Bangalore, 560 064, India

    Vaddi Damodara Reddy & Sikandar I. Mulla

  5. Department of Food Science and Biotechnology, Dongguk University-Seoul, Ilsandong-gu, Goyang, Gyeonggi, 10326, Republic of Korea

    Ganesh Dattatraya Saratale

  6. Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Science , University of Silesia in Katowice, Jagiellonska 28, 40-032, Katowice, Poland

    Urszula Guzik

  7. Graduate Program in Process Engineering , Tiradentes University, Av. Murilo Dantas, 300, Farolândia, Aracaju, Sergipe, 49032-490, Brazil

    Luiz Fernando Romanholo Ferreira

  8. Environmental Engineering, Department of Civil Engineering, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India

    Sanjay Kumar Gupta

  9. MIGAL, Galilee Research Institute, Kiryat Shmona, 11016, Israel

    Mohammed Azharuddin Savanur

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  1. Dummi Mahadevan Gurumurthy
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  2. Muhammad Bilal
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  10. Sikandar I. Mulla
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Contributions

DMG and SIM conceived and designed the study. DMG and SIM performed experimental works. DMG, LFRF, AK, GDS, MB, SKG and SIM analyzed the data. DMG, VDR and SIM wrote the paper. GDS, UG, MB, and SKG helped to revise the final draft. All the authors read and approved the final manuscript.

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Correspondence to Dummi Mahadevan Gurumurthy or Sikandar I. Mulla.

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Gurumurthy, D.M., Bilal, M., Nadda, A.K. et al. Evaluation of cell wall-associated direct extracellular electron transfer in thermophilic Geobacillus sp.. 3 Biotech 11, 383 (2021). https://doi.org/10.1007/s13205-021-02917-2

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