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Microanalysis of Two Members of Oxicam Drugs by Quenching the Fluorescence of Newly Isolated Carbonaceous Materials From Incense Ash

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Abstract

For the first time ever, useful fluorescent (FL) carbonaceous materials (CMTS) were isolated from incense ash using facile procedure on two steps; dispersion of the CMTS in water followed by filtration. The CMTS were characterized using the following techniques; dynamic light scattering (DLS), transmission electron microscopy (TEM) and Fourier transform infrared (FT-IR) spectroscopy. The CMTS exhibit excitation wavelength dependent fluorescence emission, so it can be used as a FL probe. The FL probe was employed for sensing and quantitative determination of two members of oxicam family (tenoxicam (TEN) and meloxicam (MEL)) that belongs to non-steroidal anti-inflammatory drugs (NSAIDs). The method is based on the quenching of the FL intensity of the isolated CMTS by inner filter effect mechanism (IFE). The FL intensity decreases in linear relationship with increasing the concentrations of the two cited drugs within the range of 4.0 – 30.0 µg/mL with mean percentage recoveries of 100.04 ± 0.95 and 100.07 ± 1.06 with detection limits of 1.31 µg/mL and 1.06 µg/mL for TEN and MEL, respectively. Finally, the developed sensing system was validated as per ICH guidelines and it was proved to be accurate and precise and applied successfully for quantitative determination of the two cited drugs in their capsule dosage forms with excellent percentage recoveries reaching to 97.66 ± 0.39and 98.19 ± 1.12 for TEN and MEL, respectively.

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References

  1. Unlu Z, Ay K, Tuzun C (2006) Comparison of intra-articular tenoxicam and oral tenoxicam for pain and physical functioning in osteoarthritis of the knee. Clin Rheumatol 25:54–61. https://doi.org/10.1007/s10067-005-1136-3

    Article  PubMed  Google Scholar 

  2. Bekker A, Kloepping C, Collingwood S (2018) Meloxicam in the management of post-operative pain: Narrative review. J Anaesthesiol Clin Pharmacol 34:450–457. https://doi.org/10.4103/joacp.JOACP_133_18

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Xu S, Rouzer CA, Marnett LJ (2014) Oxicams, a class of nonsteroidal anti-inflammatory drugs and beyond. IUBMB Life. 66 : 803–811. https://doi.org/10.1002/iub.1334

  4. British Pharmacopoeia Commission (2018) British Pharmacopoeia 2019 Edition

  5. Pharmacopoeia US (2018) United States pharmacopoeia and national formulary (USP 41-NF 36). InRockville, MD: United States pharmacopoeial convention

  6. Starek M, Krzek J (2009) A review of analytical techniques for determination of oxicams, nimesulide and nabumetone. Talanta 77: 925–942. https://doi.org/10.1016/j.talanta.2008.09.022

  7. Gurupadayya BM, Trinath MN, Shilpa K (2013) Spectrophotometric determination of meloxicam by sodium nitroprusside and 1,10-phenanthroline reagents in bulk and its pharmaceutical formulation. Indian J Chem Technol 111–115

  8. Tian J, Li C, Liu S, Liu Z, Yang J, Zhu J, Hu X (2014) A rapid and highly sensitive fluorimetric method for the determination of meloxicam using uranyl acetate. Anal Methods 6:5221–5226. https://doi.org/10.1039/c4ay00809j

    Article  CAS  Google Scholar 

  9. Semreen MH, Aboul-Enein HY (2010) Lc-uv method development and validation for the non steroidal anti-inflammatory agent tenoxicam. J Liq Chromatogr Relat Technol 33:720–729. https://doi.org/10.1080/10826071003609015

    Article  CAS  Google Scholar 

  10. Starek M and Krzek J (2012) TLC determination of meloxicam in tablets and after acidic and alkaline hydrolysis. Acta Pol Pharm Drug Res 225–235

  11. Tian Y, Wu X, Zhang M, Zhao L, Xiong Z, Qin F (2018) Quantitative determination of meloxicam in dog plasma by high performance liquid chromatography–tandem mass spectrometry and its application in a pharmacokinetic study. Biomed Chromatogr 32:e4228. https://doi.org/10.1002/bmc.4228

    Article  CAS  PubMed  Google Scholar 

  12. Lee HW, Ji HY, Kim HY, Lee KC, Lee HS (2009) Liquid chromatography-Tandem mass spectrometry method for the determination of meloxicam and its metabolite 5-carboxymeloxicam in human plasma. Bioanalysis 1:63–70. https://doi.org/10.4155/bio.09.10

    Article  PubMed  Google Scholar 

  13. Cox S, Bailey J, White M, Gordon K, Souza M (2017) Determination of Meloxicam in Egg Whites and Yolks Using Reverse Phase Chromatography. J Chromatogr Sci 55:610–616

    Article  CAS  Google Scholar 

  14. Leal LB, Bedor DC, Melo EK, Oliveira EJ, Santana DP (2011) Determination of meloxicam in human plasma administrated with four drugs by LC method: application to a pilot bioavailability study. Lat Am J Pharm 30

  15. Sadikoglu M, Cabuk A (2019) Voltammetric Determination of Tenoxicam in Drug Formulation at Modified Glassy Carbon Electrode. Int J Electrochem Sci 14: 4508–4519. https://doi.org/10.20964/2019.05.08

  16. Eroğlu ME, Bayraktepe DE, Polat K, Yazan Z (2018) Electro-Oxidation Mechanism of Meloxicam and Electrochemical Sensing Platform Based on Graphene Nanoparticles for its Sensing Pharmaceutical Sample. Curr Pharm Anal 15:346–354. https://doi.org/10.2174/1573412914666180402130716

    Article  CAS  Google Scholar 

  17. Šelešovská R, Hlobeňová F, Skopalová J, Cankař P, Janíková L, Chýlková J (2020) Electrochemical oxidation of anti-inflammatory drug meloxicam and its determination using boron doped diamond electrode. J Electroanal Chem 858 : 113758. https://doi.org/10.1016/j.jelechem.2019.113758

  18. Sciortino A, Cannizzo A, Messina F (2018) Carbon Nanodots: A Review—From the Current Understanding of the Fundamental Photophysics to the Full Control of the Optical Response. C - J Carbon Res 4:67. https://doi.org/10.3390/c4040067

    Article  CAS  Google Scholar 

  19. Amin N, Afkhami A, Hosseinzadeh L & Madrakian T (2018)cost-effective synthesis of carbon dots from date kernel and their application as a novel switchable fluorescence probe for sensitive assay of Zoledronic acid drug in human serum and cellular imaging. Anal Chim Acta 1030:183–193

  20. Zhang Y, Gao Z, Zhang W, Wang W, Chang J, Kai J (2018) Fluorescent carbon dots as nanoprobe for determination of lidocaine hydrochloride. Sens Act B Chem 262:928–937

  21. Ghafarloo A, Sabzi RE, Samadi N, Hamishehkar H (2020) Sensitive and selective spectrofluorimetric determination of clonazepam using nitrogen-doped carbon dots. J Photochem Photobiol A Chem 388 :112197

  22. ICH Harmonized Tripartite Guideline, Validation of analytical procedures: Text and methodology, Q2(R1), Current Step 4 Version, Parent Guid lines on Methodology. Dated November 6, 1996, Incorporated in November 2005. http://www.Ich.org/LOB/media/MEDIA417.pdf

  23. Díaz-Álvarez M, Martín-Esteban A (2020) Fluorescent carbonaceous materials isolated from cigarette ashes for the determination of iron(iii) in water samples. Anal Methods 12:3523–3529. https://doi.org/10.1039/d0ay01091j

    Article  CAS  PubMed  Google Scholar 

  24. Lecroy GE, Messina F, Sciortino A, Bunker CE, Wang P, Fernando KAS, Sun YP (2017) Characteristic Excitation Wavelength Dependence of Fluorescence Emissions in Carbon “quantum” Dots. J Phys Chem C 121:28180–28186. https://doi.org/10.1021/acs.jpcc.7b10129

    Article  CAS  Google Scholar 

  25. Sciortino A, Cayuela A, Soriano ML, Gelardi FM, Cannas M, Valcárcel M, Messina F (2017) Different natures of surface electronic transitions of carbon nanoparticles. Phys Chem Chem Phys 19:22670–22677

    Article  CAS  Google Scholar 

  26. Song Y, Zhu C, Song J, Li H, Du D, Lin Y (2017) Drug-Derived Bright and Color-Tunable N-Doped Carbon Dots for Cell Imaging and Sensitive Detection of Fe3+ in Living Cells, ACS Appl. Mater Interfaces 9:7399–7405. https://doi.org/10.1021/acsami.6b13954

    Article  CAS  Google Scholar 

  27. Wang X, Liu Y, Zhou Q, Sheng X, Sun Y, Zhou B, Zhao J, Guo J (2020) A reliable and facile fluorescent sensor from carbon dots for sensing 2, 4, 6-trinitrophenol based on inner filter effect. Sci Total Environ 720:137680

  28. Chen S, Yu YL, Wang JH (2018) Inner filter effect-based fluorescent sensing systems: A review. Anal Chim Acta 999:13–26. https://doi.org/10.1016/j.aca.2017.10.026

    Article  CAS  PubMed  Google Scholar 

  29. Chen C, Zhao D, Hu T, Sun J, Yang X (2017) Highly fluorescent nitrogen and sulfur co-doped graphene quantum dots for an inner filter effect-based cyanide sensor. Sensors Actuators B Chem 241:779–788

    Article  CAS  Google Scholar 

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Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, EinHelwanCairo, 11795, Egypt

    Mohamed Rizk, Emad Ramzy, Safaa Toubar & Marwa I. Helmy

  2. Electrochemistry and Corrosion Lab, Physical Chemistry Dept, National Research Centre, DokkiCairo, 12622, Egypt

    Nabil Abdel Ghany

Authors
  1. Mohamed Rizk
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  2. Emad Ramzy
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  3. Nabil Abdel Ghany
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  4. Safaa Toubar
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  5. Marwa I. Helmy
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by [Mohamed Mohamed Salem Rizk], [Safaa Shafike Toubar], [Marwa Ibrahim Helmy Soliman] and [Emad Ramzy Abd almalak Gadallah]. Characterization was performed by [Nabil A. Abdel Ghany]. The first draft of the manuscript was written by [Emad Ramzy Abd almalak Gadallah] and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Marwa I. Helmy.

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Highlights

• Useful fluorescent (FL) carbonaceous materials (CMTS) were isolated from incense ash and successfully employed for quantitative determination of tenoxicam (TEN) and meloxicam (MEL).

• TEN and MEL can effectively quench the fluorescence of the isolated CMTS and the mechanism was proved to be due to the inner filter effect.

• Both TEN and MEL were detected over a concentration range of 4.0 – 30.0 μg/mL.

• The developed fluorescence probe was applied successfully for quantitative determination of both drugs in their capsule dosage forms and showed reasonable selectivity in presence of various interference substances.

• The developed fluorescence probe was validated according to ICH guidelines.

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Rizk, M., Ramzy, E., Ghany, N.A. et al. Microanalysis of Two Members of Oxicam Drugs by Quenching the Fluorescence of Newly Isolated Carbonaceous Materials From Incense Ash. J Fluoresc 31, 1525–1535 (2021). https://doi.org/10.1007/s10895-021-02774-5

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  • DOI: https://doi.org/10.1007/s10895-021-02774-5

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