Abstract
Camellia chrysantha is cultivated as rare genetic resource of the family Theaceae, which mainly distributed in the South of Guangxi Zhuang Autonomous Region in China. Rhizosphere microbiota may play important roles in C. chrysantha growth and homeostasis through interaction of the plant and soil ecosystem. In order to investigate the bacterial community composition in the rhizosphere soil of three C. chrysantha cultivars under different growing conditions, Illumina MiSeq high-throughput sequencing and real-time quantitative PCR methods were used in this study. Results indicated that Proteobacteria, Acidobacteria, Chloroflexi, Actinobacteria, Bacteroidetes, and Gemmatimonadetes were the top six phyla across all the six rhizosphere soil samples. The richness and diversity of bacterial community in the rhizosphere soil from cultivar “Fangpu” grown understorey (LF) in retail planting area were significantly different from the other samples. Principal coordinate analysis revealed that bacterial community composition of the rhizosphere soil from cultivar “Fangpu” grown understorey (LF) and perennial grown undershed (PFC) was obviously separated from each other as well as the other four rhizosphere soil samples. The growing conditions displayed more influences on the bacterial community composition than plant cultivars, and close correlations were found between the soil physicochemical characters of the geographical origins and the bacterial communities. This work improves understanding of the rhizosphere bacterial community composition and the influencing soil physicochemical factors, and provides basic information for cultivation and protection of the C. chrysantha plants in China.
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References
Arafat Y, Wei XY, Jiang YH, Chen T, Saqib HAS, Lin S, Lin WX (2017) Spatial distribution patterns of root-associated bacterial communities mediated by root exudates in different aged ratooning tea monoculture systems. Int J Mol Sci 18:1727. https://doi.org/10.3390/ijms18081727
Banerjee S, Schlaeppi K, Marcel GA (2018) Keystone taxa as drivers of microbiome structure and functioning. Nat Rev Microbiol 16:567–576. https://doi.org/10.1038/s41579-018-0024-1
Bao SD (2000) Soil and agricultural chemistry analysis. China Agriculture Press, Beijing, China
Berendsen RL, Pieterse CMJ, Bakker PAHM (2012) The rhizosphere microbiome and plant health. Trends Plant Sci 17:478–486. https://doi.org/10.1016/j.tplants.2012.04.001
Clarke KR, Warwick RM (2001) Change in marine communities: an approach to statistical analysis and interpretation, 2nd edn. Plymouth, UK, Primer-E Ltd
Hou DD, Wang RZ, Gao XY, Wang K, Lin Z, Ge J, Liu T, Wei S, Chen WK, Xie RH, Yang XE, Lu LL, Tian SK (2018) Cultivar-specific response of bacterial community to cadmium contamination in the rhizosphere of rice (Oryza sativa L.). Environ Pollut 241:63–73. https://doi.org/10.1016/j.envpol.2018.04.121
Hu LF, Robert CAM, Cadot S, Zhang X, Ye M, Li BB, Manzo D, Chervet N, Steinger T, vander Heijden MGA, Schlaeppi K, Erb M, (2018) Root exudate metabolites drive plant-soil feedbacks on growth and defense by shaping the rhizosphere microbiota. Nat Commun 9:27–38. https://doi.org/10.1038/s41467-018-05122-7
Hubbard C, Li BH, McMinn R, Brock MT, Maignien L, Ewers BE, Kliebenstein D, Weinig C (2019) The effect of rhizosphere microbes outweighs host plant genetics in reducing insect herbivory. Mol Ecol 28:1801–1811. https://doi.org/10.1111/mec.14989
Hui N, Jumpponen A, Francini G, Kotze DJ, Liu XX, Romantschuk M, Strmmer R, Heikki S (2017) Soil microbial communities are shaped by vegetation type and park age in cities under cold climate. Environ Microbiol 19:1281–1295. https://doi.org/10.1111/1462-2920.13660
Jiang YJ, Li SZ, Li RP, Zhang J, Liu YH, Lv LF, Zhu H, Wu WL, Li WL (2017) Plant cultivars imprint the rhizosphere bacterial community composition and association networks. Soil Biol Biochem 109:145–155. https://doi.org/10.1016/j.soilbio.2017.02.010
Kong X, Han Z, Tai X, Jin DC, Ai S, Zheng XX, Bai ZH (2020) Maize (Zea mays L. Sp.) varieties significantly influence bacterial and fungal community in bulk soil, rhizosphere soil, and phyllosphere. Fems Microbiol Ecol 96:fiaa020. https://doi.org/10.1093/femsec/fiaa020
Larousse M, Rancurel C, Syska C, Palero F, Etienne C, Industri B, Nesme X, Bardin M, Galiana E (2017) Tomato root microbiota and Phytophthora parasitica-associated disease. Microbiome 5:56. https://doi.org/10.1186/s40168-017-0273-7
Li YC, Li Z, Li ZW, Jiang YH, Wei BQ, Lin WX (2016) Variations of rhizosphere bacterial communities in tea (Camellia sinensis L.) continuous cropping soil by high-throughput pyrosequencing approach. J Appl Microbiol 121:787–799. https://doi.org/10.1111/jam.13225
Li YC, Li ZW, Arafar Y, Lin WW, Jiang YH, Wei BQ, Lin WX (2017) Characterizing rhizosphere microbial communities in long-term monoculture tea orchards by fatty acid profiles and substrate utilization. Eur J Soil Biol 81:48–54. https://doi.org/10.1016/j.ejsobi.2017.06.008
Lin JN, Lin HY, Yang NS, Li YH, Lee MR, Chuang CH, Ho CT, Kuo SC, Way TD (2013) Chemical constituents and anticancer activity of yellow camellias against MDA-MB-231 human breast cancer cells. J Agr Food Chem 61:9638–9644. https://doi.org/10.1021/jf4029877
Marcel GA, vander Heijden, Martin H, (2016) Networking in the plant microbiome. PLOS Biol 14:e1002378. https://doi.org/10.1371/journal.pbio.1002378
Marcos MS, Bertiller MB, Olivera NL (2019) Microbial community composition and network analyses in arid soils of the Patagonian Monte under grazing disturbance reveal an important response of the community to soil particle size. Appl Soil Ecol 138:223–232. https://doi.org/10.1016/j.apsoil.2019.03.001
Mo GF, Xiao JB, Huang HS, Tang XL, Zou DF (2013) Extraction technology of total polyphenols from the leaves of camellia chrysantha (Hu) tuyama. Med Plant 4:73–75
Mori H, Maruyama F, Kato H, Toyoda A, Ohtsubo Y, Nagata Y, Fujiyama A, Tsuda M, Kurokawa K (2014) Design and experimental application of a novel non-degenerate universal primer set that amplifies prokaryotic 16s rRNA genes with a low possibility to amplify eukaryotic rRNA genes. DNA Res 21:217–227. https://doi.org/10.1093/dnares/dst052
Morita A, Yanagisawa O, Maeda S (2011) Tea plant (Camellia sinensis L.) roots secrete oxalic acid and caffeine into medium containing aluminum (Plant nutrition). Soil Sci Plant Nutr 57:796–802. https://doi.org/10.1080/00380768.2011.629176
Na XF, Xu TT, Li M, Zhou ZN, Ma SL, Wang J, He J, Jiao BZ, Ma F (2018) Variations of bacterial community diversity within the rhizosphere of three phylogenetically related perennial shrub plant species across environmental gradients. Front Microbiol 9:709. https://doi.org/10.3389/fmicb.2018.00709
Nan J, Chao LM, Ma XD, Xu DL, Mo L, Zhang XD, Zhao XP, Bao YY (2020) Microbial diversity in the rhizosphere soils of three Stipa species from the eastern Inner Mongolian grasslands. Glob Ecol Conserv 22:e00992. https://doi.org/10.1016/j.gecco.2020.e00992
Nan X, Tan GC, Wang HY (2016) Effect of biochar additions to soil on nitrogen leaching, microbial biomass and bacterial community structure. Eur J Soil Biol 74:1–8. https://doi.org/10.1016/j.ejsobi.2016.02.004
Sasse J, Martinoia E, Northen T (2018) Feed your friends: do plant exudates shape the root microbiome? Trends Plant Sci 23:25–41. https://doi.org/10.1016/j.tplants.2017.09.003
Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister E, Lesniewski RA, Oakley BB, Parks DH, Robinson CJ, Sahl JW, Thallinger GG, Van Horn DJ, Weber CF (2009) Introducing mother: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol 75:7537–7541
Tan L, Gu SS, Li S, Ren ZH, Deng Y, Liu ZH, Gong ZH, Xiao WJ, Hu QL (2019) Responses of microbial communities and interaction networks to different management practices in tea plantation soils. Sustainability 11:4428. https://doi.org/10.3390/su11164428
ter Braak CJF, Smilauer P (2012) CANOCO reference manual and user’s guide: software for ordination. Version 5. Microcomputer Power Ithaca, New York
Wei XJ, Ma JL, Wang K, Liang XJ, Lan JX, Li YJ, Li KX (2018) Early flowering induction in golden camellia seedlings and effects of paclobutrazol. HortScience 53:1849–1854. https://doi.org/10.21273/HORTSCI13676-18
Wu X, Zhang T, Zhao JN, Wang LL, Yang DL, Li G, Xiu WM (2021) Variation of soil bacterial and fungal communities from fluvo-aquic soil under chemical fertilizer reduction combined with organic materials in north China plain. J Soil Sci Plant Nut 21:349–363. https://doi.org/10.1007/s42729-020-00365-0
Yan P, Shen C, Fan LC, Li X, Zhang LP, Zhang L, Han WY (2018) Tea planting affects soil acidification and nitrogen and phosphorus distribution in soil. Agr Ecosyst Envir 254:20–25. https://doi.org/10.1016/j.agee.2017.11.015
Zhalnina K, Louie KB, Hao Z, Mansoori N, daRocha UN, Shi SJ, Cho HJ, Karaoz U, Loque D, Bowen BP, Firestone MK, Northen TR, Brodie EL (2018) Dynamic root exudate chemistry and microbial substrate preferences drive patterns in rhizosphere microbial community assembly. Nat Microbiol 3:470–480. https://doi.org/10.1038/s41564-018-0129-3
Zhang K, Zhi H, Zhang S (2017) Using an optimization algorithm to establish a network of video surveillance for the protection of Golden camellia. Ecol Inform 42:32–37. https://doi.org/10.1016/j.ecoinf.2017.08.004
Zi HY, Jiang YL, Cheng XM, Li WT, Huang XX (2020) Change of rhizospheric bacterial community of the ancient wild tea along elevational gradients in Ailao mountain. China Sci Rep-UK 10:9203. https://doi.org/10.1038/s41598-020-66173-9
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This work was supported by the Innovative Capacity Construction of Beijing Academy of Agriculture and Forestry Sciences (grant numbers KJCX20200110, KJCX20200426) and the National Key R&D Program of China (2017YFD0201102).
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Zhao, J., Liu, T., Zhang, D. et al. Bacterial Community Composition in the Rhizosphere Soil of Three Camellia chrysantha Cultivars Under Different Growing Conditions in China. J Soil Sci Plant Nutr 21, 2689–2701 (2021). https://doi.org/10.1007/s42729-021-00556-3
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DOI: https://doi.org/10.1007/s42729-021-00556-3