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Molecular and biological characterization of soybean yellow mottle mosaic virus severe strain infecting soybean in India

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Abstract

The complete nucleotide sequence and genome organization of soybean yellow mottle mosaic virus severe strain causing bright yellow mosaic, mottling and puckering symptoms in soybean (Glycine max) from India was determined. The monopartite single stranded genomic RNA is 3974 nuclotides long and has the potential to encode six viral proteins viz., p25, p83, p8, p10, p39 and p25. The SYMMV-Sb isolate differed from mungbean strain with 69 nucleotides and nine aminoacids dispersed over the various ORFs. Comparative sequence analysis revealed that SYMMV-Sb shared 98% nt sequence identity at complete genome level and 96–100% at all ORFs level with SYMMV mungbean strain from India and 71–92% identity with SYMMV Korean soybean isolate, whereas it showed very low sequence identity with other tombusviridae members (2–53%). The phylogenetic analysis showed the clustering of SYMMV-Sb along with other members of genus Gammacarmovirus. The SYMMV-Sb isolate produced chlorotic blotches, mild and veinal mottling, necrosis and puckering symptoms in various leguminous host plants. The symptomatalogy of the soybean isolate was differed from mungbean strain as earlier induced severe symptoms on soybean and mild symptoms on mungbean.

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Acknowledgements

This work was carried out under the financial support provided for the first author by Science and Engineering Research Board (DST-SERB) through Early Career Research Award ECR/2016/001016. We also thank National Phytotron Facility (NPF) for providing controlled environmental conditions to carry out the experiments.

Funding

This work was carried out under the financial support provided for the first author by Science and Engineering Research Board (DST-SERB) through Early Career Research Award ECR/2016/001016.

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Author notes

  1. Nagamani Sandra and Ankita Tripathi have contributed equally.

Authors and Affiliations

  1. Division of Seed Science and Technology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Nagamani Sandra & Ankita Tripathi

  2. Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    S. K. Lal

  3. Advanced Centre for Plant Virology, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Bikash Mandal & Rakesh Kumar Jain

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  1. Nagamani Sandra
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  2. Ankita Tripathi
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  3. S. K. Lal
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  4. Bikash Mandal
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  5. Rakesh Kumar Jain
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Contributions

NS, BM and RKJ contributed to the conceptualization, supervision and manuscript editing. NS and AT contributed to the methodology, investigation and formal analysis. SKL contributed to providing resources. NS contributed to the funding acquisition, resources, data analysis and writing the manuscript. All authors agree with the final version of the manuscript.

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Correspondence to Nagamani Sandra.

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The authors declare that they have no conflict of interest in the publication.

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No human subjects or vertebrate animals were used in this study.

Additional information

Accession numbers: GenBank Acc No: MK130864: Soybean yellow mottle mosaic virus soybean strain.

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Sandra, N., Tripathi, A., Lal, S.K. et al. Molecular and biological characterization of soybean yellow mottle mosaic virus severe strain infecting soybean in India. 3 Biotech 11, 381 (2021). https://doi.org/10.1007/s13205-021-02925-2

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