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Effect of a Heat-Stable Toxin of Yersinia pseudotuberculosis on the Functional and Phenotypic Traits of Two Types of Phagocytes in the Holothurian Eupentacta fraudatrix

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Abstract—

The impact of a heat-stable toxin of Yersinia pseudotuberculosis (HSTYp) on the markers of functional activity and phenotypes of P1- and P2-type phagocytes was studied in the holothurian Eupentacta fraudatrix. In the control, P1 and P2 phagocytes differed in the levels of apoptosis and reduced glutathione, as well as in the surface receptor binding to some plant lectins. HSTYp (0.2–2 μg/mL) caused a shift in the functional activity and phenotype of P1 phagocytes toward the prevalence of those characteristic of the P2 type, which has a lower bactericidal activity. It is supposed that HSTYp is an important factor in the reprogramming of holothurian phagocytes toward the predominance of the anti-inflammatory type, which may increase the virulence of Y. pseudotuberculosis for holothurians.

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Funding

The work was supported by Ministry of Science and Higher Education of the Russian Federation (project no. AAAA-A17-117030110038-5).

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Authors and Affiliations

  1. Il’yichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences, 690041, Vladivostok, Russia

    L. S. Dolmatova & O. A. Ulanova

  2. Somov Institute of Epidemiology and Microbiology, 690087, Vladivostok, Russia

    N. F. Timchenko

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  1. L. S. Dolmatova
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  2. O. A. Ulanova
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Correspondence to L. S. Dolmatova.

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Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Translated by E. Makeeva

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Dolmatova, L.S., Ulanova, O.A. & Timchenko, N.F. Effect of a Heat-Stable Toxin of Yersinia pseudotuberculosis on the Functional and Phenotypic Traits of Two Types of Phagocytes in the Holothurian Eupentacta fraudatrix. Biol Bull Russ Acad Sci 48, 395–406 (2021). https://doi.org/10.1134/S1062359021040051

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