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Study on removal of silver and polyethylene terephthalate from exposed radiography films using enzyme protease

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Abstract

In the proposed method, crude enzymes extracted from waste dry leaves of 15 different plants showing protease activity were used for removal of silver from exposed medical X-ray film by hydrolysis of gelatin. Optimum operating pH and temperature for crude protease extract was found to be 7 and 50 °C, respectively. The X-ray film before and after protease treatment was analysed using FTIR (Fourier-transform infrared spectroscopy) and EDS (Energy-Dispersive X-Ray Spectroscopy). The slurry obtained after smelting process analysed using EDS shows 99.8% of silver recovery. The result confirms that the enzyme extraction process recovers silver and PET (polyethylene terephthalate) in eco-friendly treatment methodology when compared to conventional chemical treatment process.

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References

  1. Khunprasert P, Grisdanurak N, Thaveesri J, Danutra V, Puttitavorn W (2008) Radiographic film waste management in Thailand and cleaner technology for silver leaching. J Clean Prod 16:28–36

    Article  Google Scholar 

  2. Handerson K (2003) The contemporary silver cycle for CIS countries: using industrial ecology to evaluate silver flows. J Young Investigators. http://legacy.jyi.org/volumes/volume9/issue1/articles/henderson.html Accessed 17 Dec 2012

  3. Kenneth MG (1983) Method and apparatus for recovering silver and plastic from used film. 1983, United States Patent. 4392889

  4. Jenkins D (1980) Film processing: equipment and procedures. In: Radiographic photography and imaging processes. Springer, Dordrecht

  5. Nakiboglu N, Toscali D, Yasa I (2001) Silver recovery from waste photographic films by an enzymatic method. Turk J Chem 25:349–353

    Google Scholar 

  6. Lee A, Liew MS (2020) Ecologically derived waste management of conventional plastics. J Mater Cycles Waste Manag 22:1–10. https://doi.org/10.1007/s10163-019-00931-4

    Article  Google Scholar 

  7. Syed S, Suresha S, Sharma LM, Syed AA (2002) Clean technology for the recovery of silver from processed radiographic films. Hydrometallurgy 63(03):277–280

    Article  Google Scholar 

  8. Hosseini SG, Pourmortazavi SM, Fathollahi M (2004) Orthogonal array design for the optimization of silver recovery from waste photographic paper. Separ sci Technol 9(8):1953–1966

    Article  Google Scholar 

  9. Khaloo SS, Torabbeigi M, Jazani RK et al (2013) Laboratory waste minimization by recovery of silver as nano-silver colloidal dispersion from waste silver chloride. J Mater Cycles Waste Manag 15:342–347. https://doi.org/10.1007/s10163-013-0123-z

    Article  Google Scholar 

  10. Canda LR, Ardelean E, Hepuț T (2018) Methods of silver recovery from radiographs—comparative study. IOP Conf Ser Mater Sci Eng. https://doi.org/10.1088/1757-899X/294/1/012007

    Article  Google Scholar 

  11. Buekens A, Yang J (2014) Recycling of WEEE plastics: a review. J Mater Cycles Waste Manag 16:415–434. https://doi.org/10.1007/s10163-014-0241-2

    Article  Google Scholar 

  12. Ishikawa H, Ishimi K, Sugiura M, Sowa A, Fujiwara N (1993) Kinetics and mechanism of enzymatic hydrolysis of gelatin layers of X-ray film and release of silver particles. J Ferment Bioeng 76:300–305

    Article  Google Scholar 

  13. Gupta R, Beg QK, Lorenz P (2002) Bacterial alkaline proteases: molecular approachesand industrial applications. Appl Microbiol Biotechnol 59:15–32

    Article  Google Scholar 

  14. Masui A, Yasuda M, Fujiwara N, Ishikawa H (2004) Enzymatic hydrolysis of gelatin Layers on used lith film using thermostable alkaline protease for recovery of Silver and PET Film. Biotechnol Prog 20(4):1267–1269

    Article  Google Scholar 

  15. Choudhary V (2013) Recovery of Silver from used X-ray films by Aspergillus versicolor protease. J Acad Ind Res 2(1):39–41

    Google Scholar 

  16. Radha KV, Arun C (2011) Recycling of exposed X-ray films and recovery of silver using Bromelain. WIT Trans Ecol Environ 142:421–430

    Google Scholar 

  17. Gaur S, Wadhwa N (2008) Alkaline protease from senesced leaves of invasive weed Lantana camara. Afr J Biotechnol 7(24):4602–4608

    Google Scholar 

  18. Sharmila S, Rebecca LJ, Das MP, Saduzzaman M (2012) Isolation and partial purification of Protease from plant leaves. J Chem Pharm Res 4(8):3808–3812

    Google Scholar 

  19. Subathra K, Jeevitha GC, Deepa R (2012) Aqueous two phase extraction of protease from neem leaves [Azadirachta indica]. Inter J Chem Sci Appl 3:346–351

    Google Scholar 

  20. Crewther WG (1951) The Precipitation of gelatin by ethanol, and its use in the estimation of proteolytic activity. Aust J Biol Sci 5(2):290–302

    Article  Google Scholar 

  21. Han Zhouxiang; Wei Jianying; Zhao Ma; Hu Jifan (2008) A method to recover silver from waste X-ray films with spent fixing bath. Hydrometallurgy 92:148–151

    Article  Google Scholar 

  22. Marokházi J, Lengyel K, Pekár S, Felföldi G, Patthy A, Gráf L, Fodor A, Venekei I (2004) Comparison of proteolytic activities produced by entomopathogenic photorhabdus bacteria: strain- and phase-dependent heterogeneity in composition and activity of four enzymes. Appl Environ Microbiol 70(12):7311–7320. https://doi.org/10.1128/AEM.70.12.7311-7320.2004

    Article  Google Scholar 

  23. Szczepanowska H, Wilson W (1998) Permanency of reprographic images on polyester film. J AM Inst Conserv 39(5):371–390

    Google Scholar 

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Authors and Affiliations

  1. Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, Andhra Pradesh, 522 502, India

    C. Arun & S. Karthikeyan

  2. Department of Chemical Engineering, National Institute of Technology, Tiruchirappalli, Tamil Nadu, 620015, India

    P. Maha Lakshmi, A. Sethupathy & P. Sivashanmugam

  3. Central University of Tamilnadu, Tiruvarur, 610001, India

    J. Rajesh Banu

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  1. C. Arun
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  2. P. Maha Lakshmi
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  4. S. Karthikeyan
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  5. P. Sivashanmugam
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  6. J. Rajesh Banu
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Correspondence to P. Sivashanmugam.

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Arun, C., Lakshmi, P.M., Sethupathy, A. et al. Study on removal of silver and polyethylene terephthalate from exposed radiography films using enzyme protease. J Mater Cycles Waste Manag 23, 1947–1954 (2021). https://doi.org/10.1007/s10163-021-01267-8

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  • DOI: https://doi.org/10.1007/s10163-021-01267-8

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