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Hymenobacter taeanensis sp. nov., radiation resistant bacterium isolated from coastal sand dune

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Abstract

An aerobic, Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, and light pink-colored bacterial strain, designated TS19T, was isolated from a sand sample obtained from a coastal sand dune after exposure to 3 kGy of gamma radiation. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that the isolate was a member of the genus Hymenobacter and was most closely related to H. wooponensis WM78T (98.3% similarity). Strain TS19T and H. wooponensis showed resistance to gamma radiation with D10 values (i.e., the dose required to reduce the bacterial population by tenfold) of 7.3 kGy and 3.5 kGy, respectively. The genome of strain TS19T consists of one contig with 4,879,662 bp and has a G + C content of 56.2%. The genome contains 3,955 protein coding sequences, 44 tRNAs, and 12 rRNAs. The predominant fatty acids of strain TS19T were iso-C15:0, summed feature 4 (iso-C17:1 I and/or anteiso-C17:1 B), summed feature 3 (C16:1 ω6c and/or C16:1 ω7c), and C16:1 ω5c. The major polar lipids were phosphatidylethanolamine, and one unidentified aminophospholipid. The main respiratory quinone was menaquinone-7. Based on the phylogenetic, physiological, and chemotaxonomic characteristics, strain TS19T represents a novel species, for which the name Hymenobacter taeanensis sp. nov. is proposed. The type strain is TS19T (= KCTC 72897T = JCM 34023T).

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Availability of data and materials

The complete genome sequence of strain TS19T generated during the current study has been deposited in GenBank with the accession codes, https://ncbi.nlm.nih.gov/nuccore/ NZ_CP053538. The 16S rRNA gene sequence also is available in the GenBank repository, https://ncbi.nlm.nih.gov/nuccore/MN911321.

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Funding

This research was supported by the Nuclear R&D program of the Ministry of Science and ICT (MSIT), Republic of Korea.

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Authors and Affiliations

  1. Radiation Research Division, Korea Atomic Energy Research Institute, Jeongeup, 56212, Republic of Korea

    Ji Hee Lee, Jong-Hyun Jung, Min-Kyu Kim & Sangyong Lim

  2. Department of Radiation Science and Technology, University of Science and Technology, Daejeon, 34113, Republic of Korea

    Jong-Hyun Jung & Sangyong Lim

  3. Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, 56212, Republic of Korea

    Han Na Choe

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  1. Ji Hee Lee
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Contributions

Conceptualization, funding acquisition, and supervision: SL; Resources: M-KK; Investigation validation, and visualization: JHL, J-HJ, HNC; Data curation and formal analysis: JHL, J-HJ; Writing-original draft: JHL; Writing-review and editing: SL.

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Correspondence to Sangyong Lim.

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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain TS19T is MN911321.

The GenBank/EMBL/DDBJ accession number for the whole genome sequence of strain TS19T is NZ_CP053538.

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Lee, J.H., Jung, JH., Kim, MK. et al. Hymenobacter taeanensis sp. nov., radiation resistant bacterium isolated from coastal sand dune. Antonie van Leeuwenhoek 114, 1585–1593 (2021). https://doi.org/10.1007/s10482-021-01624-5

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