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Comparative analysis of phenolic compounds from wild and in vitro propagated plant Thalictrum foliolosum and antioxidant activity of various crude extracts

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Abstract

Thalictrum foliolosum is an endemic herb of the temperate Himalayas and eastern China. These plants have been used for past several decades by indigenous people to treatment of fever, gastrointestinal disease, intestinal obstruction, jaundice and eyesight problems. Although a wide diversity of alkaloids and polyphenolic compounds are found in various Thalictrum species, the presence of major polyphenolic compounds in the T. foliolosum has not been much studied. In present study, both wild-collected (WC) and in vitro propagated (IVP) plants materials such as leaves and roots were used for polyphenolic compounds identification and quantification as well as different antioxidant assay. For quantitative analysis of polyphenolic compounds, extracted plant materials were subjected to HPLC–PDA. Additionally, 2, 2–diphenyl–β–picrylhydrazyl (DPPH) free radical and reducing power assay (RPA) were used to determine the antioxidant activity of various extracts. Results showed 12–13 polyphenolics compounds were identified in roots and leaves of WC and IVP plants. Spectrophotometric and HPLC analysis revealed that IVP roots had higher content of phenolics (TPC) (1489.80 ± 9.15 µg GAE/g) and flavonoid content (TFC) (783.58 ± 8.23 µg RE/g). However, strong antioxidant potential was found in chloroform fraction of IVP roots (DPPH IC50 −23.36 ± 0.60 µg/ml). Also, a positive correlation was existed between the antioxidant activity and phenolic and flavonoid content. Presence of various phenolic compounds along with the previously described alkaloids makes this Thalictrum species therapeutically important. The enrichment of polyphenolic compounds in micropropagated T. foliolosum plants provides the alternative option for various pharmaceutical industries as well as conservation.

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Acknowledgements

The first Author (Manoj K. Mishra) was supported by University Grants Commission (UGC), New Delhi, for the award of Dr. Kothari Post-Doctoral Fellowship. We thank the late professor (Dr.) Alka Srivastava (Department of Botany, University of Lucknow) for valuable guidance for the present research. We also thank the Director CSIR-NBRI, Lucknow, for providing the space for plant tissue culture room.

Funding

The first Author (Manoj K. Mishra) was supported by University Grants Commission (UGC), New Delhi, for the award of Dr. Kothari Post Doctoral Fellowship (BL/15–16/0168).

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Authors and Affiliations

  1. Department of Botany, University of Lucknow, Lucknow, Uttar Pradesh, 226007, India

    Manoj Kumar Mishra

  2. CSIR-National Botanical Research Institute, Lucknow, 226001, India

    Shatrujeet Pandey, Abhishek Niranjan & Pratibha Misra

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  1. Manoj Kumar Mishra
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  2. Shatrujeet Pandey
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  3. Abhishek Niranjan
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  4. Pratibha Misra
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Contributions

Manoj Kumar Mishra contributed to conception and designed the study, performed the experiments, acquisition of data, analyzed and interpreted the data, and wrote the manuscript; Shatrujeet Pandey performed the experiments and wrote the manuscript; Abhishek Niranjan performed HPLC analysis and interpretation the data; Pratibha Misra guided the experiment and revised the manuscript. All authors read and approved the manuscript.

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Correspondence to Manoj Kumar Mishra.

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Mishra, M.K., Pandey, S., Niranjan, A. et al. Comparative analysis of phenolic compounds from wild and in vitro propagated plant Thalictrum foliolosum and antioxidant activity of various crude extracts. Chem. Pap. 75, 4873–4885 (2021). https://doi.org/10.1007/s11696-021-01708-6

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