Abstract
Mytilus edulis embryo-larval development is often used as a bioassay to evaluate the negative impact of contaminants and environmental conditions. The toxicity criteria used in most protocols is the proportions of normal and abnormal larvae. The variety of abnormalities were described and classified, but further development of abnormal larvae remains obscure. This study aimed to reveal the possibility of correction of the morphological abnormalities after short-term exposure (48 h) in a variety of K2Cr2O7 concentrations. For this purpose, abnormal larvae, which developed under the negative influence of the series of K2Cr2O7 concentrations were transferred into clean seawater and studied after further 24 and 48 h. The obtained data, concerning changes in larval morphology, growth and survival rates during washing show that the abnormal larvae have enough capability to recover the normal D-shell structure. Moreover, restoration of the D-shell is possible even after exposure with concentration of the toxicant higher than the average effective one. The present research also pointed out that the development of larval shell (even abnormal one) is positively correlated with ability of the larvae to reconstruct D-shell and their survival rate. High mortality during washing occurs only at toxicant concentrations when no shell was formed within 48 h. Thus, the existence of the shell after 48 h exposure in the toxicant could indicate reversibility of the negative impact and help to distinguish the delay in development from its arrest.
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References
Andersen S, Grefsrud ES, Harboe T (2013) Effect of increased pCO2 level on early shell development in great scallop (Pecten maximus Lamarck) larvae. Biogeosciences 10:6161–6184. https://doi.org/10.5194/bg-10-6161-2013
ASTM (2012) E724-98, Standard guide for conducting static acute toxicity tests starting with embryos of four species of saltwater Bivalve Molluscs. PA, West Conshohocken. https://doi.org/10.1520/E0724-98
Boukadida K, Banni M, Gourves PY, Cachot J (2016) High sensitivity of embryo-larval stage of the Mediterranean mussel, Mytilus galloprovincialis to metal pollution in combination with temperature increase. Mar Environ Res 122:59–66. https://doi.org/10.1016/j.marenvres.2016.09.007
Brenko MH, Claus C, Bubic S (1977) Synergistic effects of lead, salinity and temperature on embryonic development of the mussel Mytilus galloprovincialis. Mar Biol 44:109–115. https://doi.org/10.1007/BF00386951
Calabrese A, Davis HC (1966) The pH tolerance of embryos and larvae of Mercenaria mercenaria and Crassostrea virginica. Biol Bull 131:427–436
Courtright RC, Breese WP, Krueger H (1971) Formulation of a synthetic seawater for bioassays with Mytilus edulis embryos. Water Res 5:877–888. https://doi.org/10.1016/0043-1354(71)90024-8
Gazeau F, Gattuso JP, Dawber C, Pronker AE, Peene F, Peene J, Heip CHR, Middelburg JJ (2010) Effect of ocean acidification on the early life stages of the blue mussel Mytilus edulis. Biogeosciences 7:2051–2060. https://doi.org/10.5194/bg-7-2051-2010
His E, Beiras R, Seaman MNL (1999) The assessment of marine pollution-bioassays with bivalve embryos and larvae. Adv Mar Biol 37:1–178. https://doi.org/10.1016/S0065-2881(08)60428-9
His E, Seaman MNL, Beiras R (1997) A simplification the bivalve embryogenesis and larval development bioassay method for water quality assessment. Water Res 31:351–355. https://doi.org/10.1109/acssc.2011.6190156
Iwata KS (1951) Spawning of Mytilus edulis: discharge by KCL injection. Bull Jpn Soc Sci Fish 16:393–394
Kobayashi N (1985) Marine pollution bioassay by sea urchin eggs, an attempt to enhance accuracy, II. Publ Seto Mar Biol Lab 30:213–226. https://doi.org/10.5134/176165
Kurihara H, Asai T, Kato S, Ishimatsu A (2008) Effects of elevated pCO2 on early development in the mussel Mytilus galloprovincialis. Aquat. Biol. 4:225–233. https://doi.org/10.3354/ab00109
Kurihara H, Kato S, Ishimatsu A (2007) Effects of increased seawater pCO2 on early development of the oyster Crassostrea gigas. Aquat. Biol. 1:91–98. https://doi.org/10.3354/ab00009
Leverett D, Thain J (2013) Oyster embryo-larval bioassay (revised). Oyster embryo-larval bioassay (Revised). ICES techniques in marine. Environ Sci 54:1–34
Malakhov V, Medvedeva L (1991) Embryonic development of Bivalve molluscs: normal and under heavy metals effect. Nauka Publishing, Moscow
Martin M, Osborn KE, Billig P, Glickstein N (1981) Toxicities of ten metals to Crassostrea gigas and Mytilus edulis embryos and Cancer magister larvae. Mar Pollut Bull 12:305–308. https://doi.org/10.1016/0025-326X(81)90081-3
Mendiburu FD (2019) Agricolae: statistical procedures for agricultural research. R Package Version 1.3-1. http://CRAN.R-project.org/package=agricolae
Moreira A, Freitas R, Figueira E, Ghirardini AV, Soares AMVM, Radaelli M, Guida M, Libralato G (2018) Combined effects of arsenic, salinity and temperature on Crassostrea gigas embryotoxicity. Ecotoxicol. Environ. Saf. 147:251–259. https://doi.org/10.1016/j.ecoenv.2017.08.043
Morroni L, Pinsino A, Pellegrini D, Regoli F (2018) Reversibility of trace metals effects on sea urchin embryonic development. Ecotoxicol. Environ. Saf. 148:923–929. https://doi.org/10.1016/j.ecoenv.2017.11.013
Nadella SR, Fitzpatrick JL, Franklin N, Bucking C, Smith S, Wood CM (2009) Toxicity of dissolved Cu, Zn, Ni and Cd to developing embryos of the blue mussel (Mytilus trossolus) and the protective effect of dissolved organic carbon. Comp. Biochem. Physiol. - C Toxicol. Pharmacol. 149:340–348. https://doi.org/10.1016/j.cbpc.2008.09.001
Peterson RA, Cavanaugh JE (2019) Ordered quantile normalization: a semiparametric transformation built for the cross-validation era. J Appl Stat: 1–16. https://doi.org/10.1080/02664763.2019.1630372
Schindelin J, Arganda-Сarreras I, Frise E, Kaynig V, Longair M, Pietzsch T, Preibisch S, Rueden C, Saalfeld S, Schmid B, Tinevez J, White DJ, Hartenstein V, Eliceiri K, Tomancak P, Cardona A (2012) Fiji: an open-source platform for biological-image analysis 9. https://doi.org/10.1038/nmeth.2019
R Core Team (2020) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna. https://www.R-project.org/
Ritz C, Baty F, Streibig JC, Gerhard D (2015) Dose-response analysis using R. PLoS ONE 10(12):e0146021. https://doi.org/10.1371/journal.pone.0146021
Szalaj D, De Orte MR, Goulding TA, Medeiros ID, DelValls TA, Cesar A (2017) The effects of ocean acidification and a carbon dioxide capture and storage leak on the early life stages of the marine mussel Perna perna (Linneaus, 1758) and metal bioavailability. Environ Sci Pollut Res 24:765–781. https://doi.org/10.1007/s11356-016-7863-y
USEPA (2016) Ecological Effects Test Guidelines OCSPP 850.1055: Bivalve Acute Toxicity Test (Embryo-Larval), EPA 712-C-16-006, Office of Prevention, Pesticides and Toxic Substances, October, 2016
Venables WN, Ripley BD (2002) Modern applied statistics with S, 4th edn. Springer, New York, NY, ISBN 0-387-95457-0
Waldbusser GG, Hales B, Langdon CJ, et al (2015) Ocean acidification has multiple modes of action on bivalve larvae. PLoS ONE 10. https://doi.org/10.1371/journal.pone.0128376
Wickham H (2016) ggplot2: elegant graphics for data analysis. Springer-Verlag, New York
Yaroslavtseva LM, Sergeeva EP (2005) Effect of copper ions on early developmental stages of the mussel Mytilus trossulus (Bivalvia). Russ J Mar Biol 31:225–231. https://doi.org/10.1007/s11179-005-0077-7
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The authors are grateful to the staff of the White Sea Biological Station of the Lomonosov Moscow State University for the laboratory and equipment provided for this study.
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Saidov, D.M., Kosevich, I.A. Rehabilitation of Mytilus edulis larvae abnormalities induced by K2Cr2O7 in short-term experiments. Ecotoxicology 30, 1242–1250 (2021). https://doi.org/10.1007/s10646-021-02441-2
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DOI: https://doi.org/10.1007/s10646-021-02441-2