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Development of CRISPR_Cas9 genome editing system and its application in rice molecular breeding

  • Special Issue on Genome Editing
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Abstract

Gene editing is a new genetic engineering technology that uses sequence-specific nucleases to insert, delete, or replace nucleic acid base(s) of the target gene, or introduce exogenous DNA sequences at a specific site in the genome of an organism to achieve the desired modification. Currently, the CRISPR/Cas system is the most widely used genome editing technology because it is relatively simple with high editing efficiency. In this review, we discuss the CRISPR/Cas system’s application and its current limitations in rice breeding. Also, we elaborate on possible improved methods regarding its mechanism and development history.

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Acknowledgements

This research project is supported by National Natural Science Foundation of China (31000703 and 31360329), Guangxi Science and Technology Projects (AA17204049, AA18221043 and AA17204050-2).

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Authors and Affiliations

  1. Guangxi Talent Highland of High Quality Rice Breeding Research, Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, 530007, China

    Zengfeng Ma, Minyi Wei, Yuexiong Zhang, Gang Qin, Chi Liu, Zhenjing Li & Dahui Huang

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  1. Zengfeng Ma
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  2. Minyi Wei
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  3. Yuexiong Zhang
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  4. Gang Qin
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  5. Chi Liu
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Correspondence to Dahui Huang.

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Editor: Prakash Lakshmanan

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Ma, Z., Wei, M., Zhang, Y. et al. Development of CRISPR_Cas9 genome editing system and its application in rice molecular breeding. In Vitro Cell.Dev.Biol.-Plant 57, 700–708 (2021). https://doi.org/10.1007/s11627-021-10203-2

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