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Comparative Study of ESBL Production Among Uropathogenic Escherichia coli Clinical Isolates from Pre- and Post-menopausal Women in Egypt

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Abstract

Urinary tract infection (UTI) is regarded one of the most frequent bacterial infections in women. Accordingly, the aim of the current study was to determine the prevalence of extended-spectrum beta-lactamase (ESBL), as well as the degree of antimicrobial resistance among premenopausal (n = 44) and postmenopausal (n = 49) women suffering from uncomplicated UTI. Urinary samples (n = 93) collected from women with UTI were tested for their antimicrobial sensitivity and assessed for ESBL production by both phenotypic and genotypic methods. Phenotypically, the presence of ESBL was observed in 64 isolates, while polymerase chain reaction detected ESBL-encoding genes in 57 isolates. The CTX-M gene was the most predominant (51.6%), followed by TEM (46.2%), and the SHV gene (17.2%). Surprisingly, all ESBL-producing Escherichia coli isolates were multidrug-resistant (MDR). To the best of our knowledge, this is the first study conducted in Egypt showing significant correlation between ESBL production, multidrug resistance and menopausal state in women. The results demonstrate alarming signal for the dissemination of ESBL genes among uropathogenic E. coli that are MDR in Egypt.

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Acknowledgements

The author thanks Pharos University in Alexandria and Medical technology center for their technical support.

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  1. Department of Microbiology and Immunology, Faculty of Pharmacy, Pharos University in Alexandria, Mahmoudeya Canal, Alexandria, 21311, Egypt

    Ghada Hani Ali & Marwa A. Yakout

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GHA and MAY designed and performed the experiments. GHA wrote the manuscript.

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Correspondence to Ghada Hani Ali.

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Regarding ethical clarification required concerning the isolation of bacterial isolates form clinical specimens, this was carried out by nurses and technicians in the hospitals not by us. We only collected the subcultured isolates from the microbiology laboratories in these hospitals. Privacy and confidentiality of all patients’ information was anonymized. Therefore, no need for ethical approval or consent.

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Ali, G.H., Yakout, M.A. Comparative Study of ESBL Production Among Uropathogenic Escherichia coli Clinical Isolates from Pre- and Post-menopausal Women in Egypt. Curr Microbiol 78, 3516–3525 (2021). https://doi.org/10.1007/s00284-021-02599-2

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