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Antagonism and biocontrol of walnut blight by sweet osmanthus endophytic bacterium OFE17

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Abstract

Walnut blight caused by Xanthomonas arboricola pv. juglandis (Xaj) is an important bacterial disease for walnut production worldwide. The objective of the present study was to characterize one endophytic bacterium, namely OFE17 from Osmanthus fragrans leaves, evaluate its potential biocontrol efficiency against the disease, and identify the underlying probable mechanisms of its function. Based on morphology, biochemical and physiological characteristics, 16S-rDNA and gyrB sequences, and antibiotic production genes, the endophyte OFE17 was tentatively identified as Bacillus sp. A disease control efficiency of up to 68.69% was observed through a biocontrol test on detached immature walnut fruits under controlled conditions. OFE17 can produce protease, cellulase, amylase, siderophores, and demonstrates phosphate dissolving ability. However, the OFE17 is unable to produce extracellular lipase, IAA (indoleacetic acid), and has no nitrogen fixation capability. The active compounds of OFE17 were primarily non-protein compounds, and the optimum organic extraction solvent was chloroform. Through specific PCR detection, it contains the genes ituA and ituD which play a key role in active compound synthesis of iturin A synthetase. This study added a promising biocontrol agent candidate for the disease control and laid a foundation for further exploration.

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Data availability

The sequences were deposited in NCBI. The strains are available for scientific research use only by request from the corresponding author Dr. Fu’s consent.

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Funding

The work was supported by the Central-guided Local Project of Science & Technology Development (No.2019ZYYD045).

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Authors and Affiliations

  1. Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables, College of Life Science and Technology, Hubei Engineering University, Xiaogan, 432000, Hubei, China

    Benzhong Fu, Lulu Zou & Lihua Wang

  2. Department of Plant Pathology & Microbiology, Iowa State University, Ames, IA, 50011, USA

    Benzhong Fu & Conard Lee

  3. Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, Montana, 59717, USA

    Benzhong Fu

  4. Wuhan Seqhealth Technology Co, Ltd, Wuhan, 430073, Hubei, China

    Lulu Zou

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  1. Benzhong Fu
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  2. Lulu Zou
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B. Fu conceived and designed the work, L. Zou and B. Fu carried out the experiment. B. Fu and C. Lee prepared the manuscript, L. Wang supported the research and revised the manuscript, and all authors read and approved the final manuscript.

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Correspondence to Benzhong Fu.

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Fu, B., Zou, L., Lee, C. et al. Antagonism and biocontrol of walnut blight by sweet osmanthus endophytic bacterium OFE17. J Plant Pathol 103, 1243–1252 (2021). https://doi.org/10.1007/s42161-021-00901-6

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