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A novel anellovirus from hospitalized neonates

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Abstract

Here, using viral metagenomics combined with conventional PCR, the complete genome sequence of a novel anellovirus (named anel-ch-zj and GenBank no. MT157223) from nasopharynx secretion specimens from hospitalized neonates was determined, and the deduced amino acid sequence of its ODF1 protein was found to be only 33.19%-39.33% identical to those of related anelloviruses with sequences available in the GenBank database, suggesting that it represents a putative new genus within the family Anelloviridae. PCR screening of 135 samples (including 45 nasopharynx secretion, 45 blood, and 45 fecal specimens collected from 45 individual hospitalized neonates) indicated that two nasopharynx secretion, two blood, and four fecal samples were positive for anel-ch-zj. Further PCR screening of 50 blood samples, 115 fecal samples, and 396 nasopharynx secretions collected from hospitalized children 1-5 years old did not yield any positive results. Whether this novel anellovirus detected in neonates is associated with specific diseases needs future investigation.

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Data availability

The complete genome of the novel anellovirus determined in this study was deposited in the GenBank database with the accession no. MT157223. The raw reads generated by NGS from nasopharynx secretions of neonates were deposited in the Sequence Read Archive of the GenBank database, and the accession nos. are SRX7070391, SRX7070404, SRX7070494, SRX7070535, and SRX7070536.

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Acknowledgements

This work was supported by the National Key Research and Development Programs of China No. 2017YFC1200201, Jiangsu Provincial Key Research and Development Projects No. BE2017693. We thank all participants in the study.

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Authors and Affiliations

  1. Department of Pediatrics, Affiliated Hospital of Jiangsu University, Zhenjiang, 212000, Jiangsu, People’s Republic of China

    Hong Li, Jinquan Lv, Shixing Yang & Hongyan Lu

  2. School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, People’s Republic of China

    Hong Li, Yuqing Xiao, Heteng Zhang, Xu Chen & Wen Zhang

  3. International Genome Center, Jiangsu University, Zhenjiang, 212013, Jiangsu, People’s Republic of China

    Hongyan Lu & Wen Zhang

  4. School of Medicine, Jiangsu University, 310 Xuefu Road, Zhenjiang, 202103, Jiangsu, People’s Republic of China

    Wen Zhang

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  1. Hong Li
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  2. Yuqing Xiao
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  3. Heteng Zhang
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  4. Xu Chen
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  5. Jinquan Lv
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  7. Hongyan Lu
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  8. Wen Zhang
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Corresponding authors

Correspondence to Hongyan Lu or Wen Zhang.

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The authors declare that they have no conflict of interest.

Ethical approval

This study did not include experiments with human participants or animals performed by any of the authors.

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Informed consent was provided by the parents or guardians of the children included in the study.

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Handling Editor: Zhongjie Shi.

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Cite this article

Li, H., Xiao, Y., Zhang, H. et al. A novel anellovirus from hospitalized neonates. Arch Virol 166, 2623–2625 (2021). https://doi.org/10.1007/s00705-021-05155-2

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  • DOI: https://doi.org/10.1007/s00705-021-05155-2

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