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MiR-99b-5p suppressed proliferation of human osteoblasts by targeting FGFR3 in osteoporosis

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Abstract

Osteoporosis is a common skeletal disease characterized by reduced bone mass partially caused by an imbalance between bone resorption and formation. Considering the potential role of microRNAs (miRNAs) in osteoporosis, we attempted to identify deregulated miRNA that participates in the pathogenesis of osteoporosis. We analyzed online datasets for differentially expressed miRNAs and predicted deregulated miRNA target genes, applied these genes for signaling pathway enrichment annotation, and selected the possible miR-99b-5p/FGFR3 axis. Within osteoporosis bone tissues, miR-99b-5p was upregulated and FGFR3 was downregulated. miR-99b-5p overexpression inhibited osteoblast proliferation and osteogenesis-related genes expression, whereas FGFR3 overexpression exerted opposite effects upon the proliferation of osteoblasts and osteogenesis-related genes expression. By direct targeting, miR-99b-5p inhibited FGFR3 expression. Moreover, FGFR3 silencing significantly reversed the roles of miR-99b-5p inhibition in the proliferation of osteoblasts and osteogenesis-related genes expression. In conclusion, we identify a deregulated miRNA/mRNA axis in osteoporosis and osteogenic differentiation, namely the miR-99b-5p/FGFR3 axis; through targeting FGFR3, miR-99b-5p inhibits osteoblast proliferation and activity, which might subsequently affect the bone formation in osteoporosis progression.

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Acknowledgements

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Funding

This study was supported by the fund of Technological Innovation Guidance Plan of Science and Technology Department of Hunan Province (2018SK52507).

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Authors and Affiliations

  1. Department of Orthopaedics, The Second Xiangya Hospital, Central South University, Changsha, 410001, Hunan, China

    Muliang Ding, Bo Liu, Xia Chen, Zhengxiao Ouyang & Dan Peng

  2. Department of Emergency Medicine, The Second Xiangya Hospital, Central South University, Changsha, 410001, Hunan, China

    Yu Zhou

  3. Emergency Medicine and Difficult Diseases Institute, Central South University, Changsha, 410001, Hunan, China

    Yu Zhou

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  1. Muliang Ding
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  2. Bo Liu
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  3. Xia Chen
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  4. Zhengxiao Ouyang
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  6. Yu Zhou
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Correspondence to Yu Zhou.

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The investigation was performed with the approval of the Research Ethics Committee of the Second Xiangya Hospital (2020 (Yan110)).

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Ding, M., Liu, B., Chen, X. et al. MiR-99b-5p suppressed proliferation of human osteoblasts by targeting FGFR3 in osteoporosis. Human Cell 34, 1398–1409 (2021). https://doi.org/10.1007/s13577-021-00567-3

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  • DOI: https://doi.org/10.1007/s13577-021-00567-3

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