Abstract
MicroRNAs (miRNAs) are implicated in various cancer-relevant cellular processes, including cell proliferation, migration, invasion, and angiogenesis. However, the function of miRNAs in hepatocellular cancer (HCC) has not been fully clarified. This study aimed to investigate the role of miR-1178-3p in HCC metastasis and try to reveal the potential mechanism. In the present study, we found that miR-1178-3p was down-regulated, while TBL1XR1 was up-regulated in HCC cancer tissues by bioinformatics analysis and RT-PCR. We further confirmed the connection of miR-1178-3p and TBL1XR1 using dual-luciferase reporter (DLR) assay. Moreover, gain- and loss-of-function experiments demonstrated that overexpress miR-1178-3p inhibited cell proliferation, migration, and invasion in HCC cells and reduced the xenograft tumor growth and angiogenesis by regulating the TBL1XR1/PI3K/Akt axis. Our results indicated that the novel identified miR-1178-3p functions as a tumor suppressor in HCC through regulating TBL1XR1/PI3K/Akt pathway, and these findings could be a valid molecular target for liver cancer therapy.
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The data sets and supporting materials for the current study are available from the corresponding author on reasonable request.
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The present study was supported by the National Natural Science Foundation of China (No. 81373169).
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Contributions: (I) Conception and design: H Liu; (II) Administrative support: H Liu; (III) Provision of study materials or patients: Q Pan; (IV) Collection and assembly of data: ZG Shao and YJ Zhang; (V) Data analysis and interpretation: Q Pan; (VI) Manuscript writing: Q Pan; (VII) Final approval of manuscript: All authors.
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Written informed consent was obtained from all patients, and the study protocol was approved by the Ethics Committee of First Hospital of China Medical University, the approval number: AF-OG-03-1.0-02. All animal research were carried out following approval by the Institutional Animal Care and Use Committee of First Hospital of China Medical University.
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Pan, Q., Shao, Z., Zhang, Y. et al. MicroRNA-1178-3p suppresses the growth of hepatocellular carcinoma by regulating transducin (beta)-like 1 X-linked receptor 1. Human Cell 34, 1466–1477 (2021). https://doi.org/10.1007/s13577-021-00565-5
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DOI: https://doi.org/10.1007/s13577-021-00565-5