Abstract
A method is proposed of fabricating tubular constructs from tissue spheroids (conglomerates of cells up to 200 μm in size) in a nutrient fluid using the acoustic radiation force. The source of the acoustic field is a hollow piezoceramic cylinder with a resonant frequency of 800 kHz. Keeping the obtained structure at 37°C for 24 h fuses the spheroids into a solid tubular viable tissue construct.
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Funding
This work was supported by the Russian Foundation for Basic Research, projects no. 20-32-90093, 20-02-00139, and 18-29-11076; and by the Basis Foundation for the Advancement of Theoretical Physics and Mathematics.
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Translated by K. Gumerov
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Krokhmal, A.A., Sapozhnikov, O.A., Koudan, E.V. et al. Creating Tubular Structures from Tissue Spheroids via the Acoustic Radiation Force. Bull. Russ. Acad. Sci. Phys. 85, 681–685 (2021). https://doi.org/10.3103/S1062873821060137
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DOI: https://doi.org/10.3103/S1062873821060137