Abstract
Strain JC669T was isolated from a floating island of Loktak lake, Manipur, India and shares the highest 16S rRNA gene sequence identity with Aquisphaera giovannonii OJF2T. The novel strain is aerobic, Gram negative, light pink-coloured, non-motile, NaCl intolerant and spherical to oval-shaped. It grows in the form of single cells or aggregates and possibly forms structures which appear like fruiting bodies. Strain JC669T grows well up to pH 9.0.The isolate produces MK-6 as respiratory quinone, C18:1ω9c, C16:0 and C18:0 as major fatty acids and phosphatidylcholine, an unidentified amino lipid, an unidentified choline lipid (UCL) and six additional unidentified lipids (UL1, 2, 3, 4, 5, 6) as polar lipids. Strain JC669T has a large genome size of 10.04 Mb and the genomic G + C content was 68.5 mol%. The genome contained all genes essential for lycopene related carotenoid biosynthesis. The polyphasic analysis of its phylogenetic position, morphological, physiological and genomic features supports the classification of strain JC669T as a novel species of the genus Aquisphaera, for which we propose the name Aquisphaera insulae sp. nov. Strain JC669T (= KCTC 72672T = NBRC 114306T) is the type strain of the novel species.
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Abbreviations
- NCBI:
-
National Centre for Biotechnology Information
- gANI:
-
Genome average nucleotide identity
- AAI:
-
Average amino acid identity
- dDDH:
-
Digital DNA–DNA hybridization
- HPLC:
-
High performance liquid chromatography
- KCTC:
-
Korean collection for type cultures
- NBRC:
-
Biological Resource Centre
- PGAP:
-
Prokaryotic genome annotation pipeline
- EMBL:
-
European Molecular Biology Laboratory
- PVC:
-
Planctomycetes, Verrucomicrobia and Chlamydiae
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Acknowledgements
Gaurav and Ramana thank DBT, New Delhi for the award of SRF and TATA innovation fellowship, respectively. Sasikala, Dhanesh, Shabbir and Jagadeeshwari thank UGC for Mid-career award, RA, SRF and TEQIP awards for JRF, respectively. We also thank Prof. Bernhard Schink for providing the species name and etymology for strain JC669Tand Prof. T. Radhakrishnan and Dr. Senthilnathan of the Department of Chemistry for providing “Sporotan”.
Funding
This work was supported by a grant from the Department of Biotechnology, Government of India, with sanction No. BT/HRD/35/01/02/2015. Financial support received from the Council for Scientific and Industrial Research (CSIR), New Delhi, UGC and AICTE is acknowledged. Infrastructure facilities funded by DST-FIST, UGC-SAP (DRS), TEQIP and AICTE are acknowledged.
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KL performed sample collection, KG performed media optimization and polar lipid analysis, KG and KL isolated the strain, performed the initial cultivation, strain deposition and strain characterization; KG and DK performed the electron microscopic analysis; JU performed the genomic and phylogenetic analysis; SA performed and analyzed the data for polyamines; GMK and KG performed genome mining for genes related to sporulation; KG and KL wrote the manuscript; Ramana and Sasikala supervised the study and contributed to text preparation and revised the manuscript. All authors read and approved the final version of the manuscript.
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The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence of the strain JC669T is LR782133. The NCBI accession for the whole genome shotgun sequence of strain JC669T is JAALJH000000000.
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Kumar, G., Lhingjakim, K.L., Uppada, J. et al. Aquisphaera insulae sp. nov., a new member in the family Isosphaeraceae, isolated from the floating island of Loktak lake and emended description of the genus Aquisphaera. Antonie van Leeuwenhoek 114, 1465–1477 (2021). https://doi.org/10.1007/s10482-021-01615-6
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DOI: https://doi.org/10.1007/s10482-021-01615-6