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Infrared Emission Spectroscopy for Investigation of Biological Molecules in Aqueous Solutions

  • AQUEOUS SOLUTIONS IN BIOLOGICAL SYSTEMS
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Abstract

Examples are given of using infrared emission spectroscopy to analyze the structure of biological molecules in aqueous solutions. Instead of sample heating commonly used in emission spectroscopy, it is proposed to use a cold background for screening environmental thermal emission. Emission spectra are measured for three types of molecular biological objects in aqueous solutions: protein (bovine serum albumin 1 mg/mL in H2O), phospholipid (1,2-dimiristoylphosphatidylcholine 1 mg/mL in D2O) in two phase states (gel at 10°С and liquid crystal at 35°С), and nucleotide (adenosine-5'-triphosphate 1 mg/mL in H2O). Characteristic bands are obtained for each of the samples. Higher sensitivity and wider application of infrared emission spectroscopy with a cold background in comparison to that with sample heating are discussed.

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Funding

This work was performed on the equipment of the Optical Microscopy and Spectrophotometry Core Facility of the Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences” (http://www.ckp-rf.ru/ckp/670266/).

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Authors and Affiliations

  1. Institute of Cell Biophysics, Russian Academy of Sciences, Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, 142290, Pushchino, Moscow oblast, Russia

    N. V. Penkov

  2. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia

    N. A. Penkova

Authors
  1. N. V. Penkov
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  2. N. A. Penkova
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Correspondence to N. V. Penkov.

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Translated by M. Potapov

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Penkov, N.V., Penkova, N.A. Infrared Emission Spectroscopy for Investigation of Biological Molecules in Aqueous Solutions. Phys. Wave Phen. 29, 164–168 (2021). https://doi.org/10.3103/S1541308X21020102

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  • DOI: https://doi.org/10.3103/S1541308X21020102

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