Abstract
The results of identifying the vaccinia virus with the use of nanowire biosensors manufactured on the basis of silicon-on-insulator (SOI) films were presented. In our experiments, the vaccinia virus, the LIVP strain from the collection of the State Research Center of Virology and Biotechnology VECTOR of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, and the rabbit blood serum containing specific polyclonal antibodies to the vaccinia virus were used. As shown by our studies, the polyvalent blood serum was electrically neutral at the sensor surface–viral suspension phase interface, the vaccinia virus was positively charged, and polyvalent blood serum–vaccinia virus vaccine complexes had a negative effective charge.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
This Is What Happened: Program of Global Eradication of Smallpox in Memoirs of Participants, Ed. by S. S. Marennikova (TsERIS, Novosibirsk, 2011).
G. A. Shchelkunova and S. N. Shchelkunov, ‘‘40 years without smallpox,’’ Acta Nat. 9 (4) 4–12 (2017). https://doi.org/10.32607/20758251-2017-9-4-4-12
S. N. Shchelkunov, ‘‘Is re-emerging of smallpox possible?,’’ Mol. Med., No. 4, 36–41 (2011).
A. N. Sergeev, A. S. Safatov, V. M. Generalov, et al., ‘‘Highly patogenic bird influenza in Russian and abroad: control and prevention strategy,’’ Probl. Osobo Opasnykh Infektsii, No. 1, 5–11 (2006).
O. V. Naumova, B. I. Fomin, D. A. Nasimov, N. V. Dudchenko, S. F. Devyatova, E. D. Zhanaev, V. P. Popov, A. V. Latyshev, A. L. Aseev, Yu. D. Ivanov, and A. I. Archakov, ‘‘SOI nanowires as sensors for charge detection,’’ Semicond. Sci. Technol. 25, 055004 (2010). https://doi.org/10.1088/0268-1242/25/5/055004
O. V. Naumova and B. I. Fomin, ‘‘Optimization of the response of nanowire biosensors,’’ Optoelectron., Instrum. Data Process. 52, 434–437 (2016). https://doi.org/10.3103/S8756699016050034
Yu. D. Ivanov, K. A. Malsagova, T. O. Pleshakova, I. D. Shumov, A. L. Kaysheva, V. P. Popov, O. V. Naumova, B. I. Fomin, D. A. Nasimov, A. V. Latyshev, A. L. Aseev, V. Yu. Tatur, N. D. Ivanova, G. M. Konovalova, and A. I. Archakov, ‘‘Registration of the protein in the serum with a field-effect nanotransistor biosensor,’’ Pathol. Physiol. Exper. Ther. 60 (1) 94–98 (2016).
V. M. Generalov, O. V. Naumova, B. I. Fomin, S. A. P’yankov, I. V. Khlistun, A. S. Safatov, B. N. Zaitsev, E. G. Zaitseva, and A. L. Aseev, ‘‘Detection of ebola virus VP40 protein using a nanowire SOI biosensor,’’ Optoelectron., Instrum. Data Process. 55, 618–622 (2019). https://doi.org/10.3103/S875669901906013X
Fundamental Virology, Ed. by B. N. Fields and D. M. Knipe (Raven Press, New York, 1989).
M. S. Makowski and A. Ivanisevic, ‘‘Molecular analysis of blood with micro-/nanoscale field-effect-transistor biosensors,’’ Small 7, 1863–1875 (2011). https://doi.org/10.1002/smll.201100211
F. Yang and G.-J. Zhang, ‘‘Silicon nanowire-transistor biosensor for study of molecule-molecule interactions,’’ Rev. Anal. Chem. 33, 95–110 (2014). https://doi.org/10.1515/revac-2014-0010
K. A. Malsagova, Yu. D. Ivanov, T. O. Pleshakova, A. F. Kozlov, N. V. Krohin, A. L. Kaysheva, I. D. Shumov, V. P. Popov, O. V. Naumova, B. I. Fomin, and D. A. Nasimov, ‘‘SOI-nanowire biosensor for the detection of D-NFAT 1 protein,’’ Biochemistry (Moscow) Suppl. Ser. B: Biomed. Chem. 8, 220–225 (2014). https://doi.org/10.1134/S199075081403010X
J. Park, H. H. Nguyen, A. Woubit, and M. Kim, ‘‘Applications of field-effect transistor (FET)-type biosensors,’’ Appl. Sci. Converg. Technol. 23, 61–71 (2014). https://doi.org/10.5757/ASCT.2014.23.2.61
P. Cherpillod, M. Schibler, G. Vieille, S. Cordey, A. Mamin, P. Vetter, and L. Kaiser, ‘‘Ebola virus disease diagnosis by real-time RT-PCR: A comparative study of 11 different procedures,’’ J. Clin. Virol. 77. P. 9–14 (2016). https://doi.org/10.1016/j.jcv.2016.01.017
R. A. Maksyutov, ‘‘Complex approach to species-specific detection of vaccinia virus,’’ Probl. Osobo Opasnykh Infektsii, No. 4, 60–63 (2016). https://doi.org/10.21055/0370-1069-2016-4-60-63
A. G. Poltavchenko, A. V. Ersh, O. S. Taranov, S. N. Yakubitskiy, and P. V. Filatov, ‘‘Rapid immunochemical method for the detection of orthopoxviruses (Orthopoxvirus, Chordopoxvirinae, Poxviridae),’’ Probl. Virol. 64, 291–297 (2019). https://doi.org/10.36233/0507-4088-2019-64-6-291-297
K.-I Chen, B.-R. Li, and Y.-T. Chen, ‘‘Silicon nanowire field-effect transistor-based biosensors for biomedical diagnosis and cellular recording investigation,’’ Nano Today 6, 131–154 (2011). https://doi.org/10.1016/j.nantod.2011.02.001
GOST R (State Standard) 54500.3-2011: Uncertainty of Measurement. Part 3. Guide to the Expression of Uncertainty in Measurement, 2011.
ACKNOWLEDGMENTS
The authors are grateful to D. V. Shcheglov for the organization support of this study and O. S. Taranov for the granted electron photos of vaccinia virus.
Funding
The preparation of samples was carried out within state task of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing. The manufacturing of sensors and the indication of viruses were carried out in the Institute of Semiconductor Physics of the Siberian Branch of the Russian Academy of Sciences under support from the Russian Foundation for Basic Research (grant no. 18-29-02091).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated by E. Glushachenkova
About this article
Cite this article
Generalov, V.M., Naumova, O.V., P’yankov, S.A. et al. Identifying the Vaccinia Virus with the Use of a Nanowire Silicon-on-Insulator Biosensor. Optoelectron.Instrument.Proc. 57, 37–43 (2021). https://doi.org/10.3103/S8756699021010040
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.3103/S8756699021010040