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Metabolic accumulation and related synthetic genes of O-acetyl groups in mannan polysaccharides of Dendrobium officinale

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Abstract

Mannan polysaccharides (MPs), which contain substituted O-acetyl groups in their backbone, are abundant in the medicinal plant Dendrobium officinale. Acetyl groups can influence the physiological and biochemical properties of polysaccharides, which mainly accumulate in the stems of D. officinale at four developmental stages (S1–S4), showing an increasing trend and a link with water-soluble polysaccharides (WSPs) and mannose. The genes coding for enzymes that catalyze O-acetyl groups to MPs are unknown in D. officinale. The TRICHOME BIREFRINGENCE-LIKE (TBL) gene family contains TBL and DUF231 domains that can transfer O-acetyl groups to various polysaccharides. Based on an established D. officinale genome database, 37 DoTBL genes were identified. Analysis of cis-elements in the promoter region showed that DoTBL genes might respond to different hormones and abiotic stresses. Most of the genes with MeJA-responsive elements were upregulated or downregulated after treatment with MeJA. qRT-PCR results demonstrated that DoTBL genes had significantly higher expression levels in stems and leaves than in roots. Eight DoTBL genes showed relatively higher expression at S2–S4 stages, which showed a link with the content of WSPs and O-acetyl groups. DoTBL35 and its homologous gene DoTBL34 displayed the higher mRNA level in different organs and developmental stages, which might participate in the acetylation of MPs in D. officinale. The subcellular localization of DoTBL34 and DoTBL35 reveals that the endoplasmic reticulum may play an important role in the acetylation of MPs.

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Data availability

The datasets used during the current study are available from corresponding author on reasonable request.

Abbreviations

ABA:

Abscisic acid

Axy9:

Altered xyloglucan9

GA:

Gibberellin

HPLC:

High performance liquid chromatography

MP:

Mannan polysaccharide

MeJA:

Methyl jasmonate

NJ:

Neighborhood-joining

ORF:

Open reading frame

PMP:

1-Phenyl-3-methyl-5-pyrazolone

qRT-PCR:

Quantitative real-time polymerase chain reaction

RWA:

Reduced wall acetylation

SA:

Salicylic acid

SDS:

Sodium dodecyl sulfate

TBL:

Trichome birefringence-like

WSP:

Water-soluble polysaccharide

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Acknowledgements

We thank Ming-zhi Li and Nanjing Genepioneer Co. Ltd. for the hummersearch analysis.

Funding

This research was funded by the project “Cultivation of new varieties of Dendrobium officinale in Guangdong Province” (Y 334041001).

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Authors and Affiliations

  1. Key Laboratory of South China Agricultural Plant Molecular Analysis of Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China

    Can Si, Chunmei He, Zhenming Yu & Jun Duan

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Can Si

  3. Independent Researcher, P. O. Box 7, Miki-cho post office, Ikenobe 3011-2, Miki-cho, Kita-gun, Kagawa-ken, 761-0799, Japan

    Jaime A. Teixeira da Silva

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  1. Can Si
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  2. Chunmei He
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Contributions

JD designed the research. CS wrote the manuscript and performed all the experiments. CH and ZY helped to collect and analyze the data. CH helped to draw the figures. JATdS aided with data analysis, interpretation of results, and co-wrote the manuscript. All authors read and approved the final manuscript.

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Correspondence to Jun Duan.

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Si, C., He, C., Teixeira da Silva, J.A. et al. Metabolic accumulation and related synthetic genes of O-acetyl groups in mannan polysaccharides of Dendrobium officinale. Protoplasma 259, 641–657 (2022). https://doi.org/10.1007/s00709-021-01672-8

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