In this study, a simple, one-step organic molecule 3-aminopropyltriethoxysilane (APTES) functionalized surface plasmon resonance (SPR) sensor was developed. APTES as an organic ligand immobilized on the SPR sensor chip was used to form the Meisenheimer complex with 2,4,6-trinitrotoluene (TNT). The results of using the APTES-based SPR sensor chip show a highly selective and sensitive (ppb level: parts per billion) detection of TNT explosive. The sensor is expected to have potential for application in the fast screening of the TNT explosive.