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Nonspecific Synthesis in the Reactions of Isothermal Nucleic Acid Amplification

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Abstract

The review focuses on the main factors involved in the formation of nonspecific products in isothermal nucleic acid amplification, such as mispriming, ab initio DNA synthesis, and additional activities of DNA polymerases, and discusses approaches to prevent formation of such nonspecific products in LAMP, RPA, NASBA, RCA, SDA, LSDA, NDA, and EXPAR.

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Abbreviations

EXPAR:

exponential amplification reaction

LAMP:

loop-mediated isothermal amplification

NASBA:

nucleic acid sequence-based amplification

RCA:

rolling circle amplification

RPA:

recombinase polymerase amplification

SDA:

strand displacement amplification

SSB:

single-stranded DNA binding protein

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Funding

This work was supported by the State Budget Project no. 075-00845-20-01.

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  1. Institute of Protein Research, Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Russia

    Nadezhda V. Zyrina

  2. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Russia

    Nadezhda V. Zyrina & Valeriya N. Antipova

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Correspondence to Valeriya N. Antipova.

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Zyrina, N.V., Antipova, V.N. Nonspecific Synthesis in the Reactions of Isothermal Nucleic Acid Amplification. Biochemistry Moscow 86, 887–897 (2021). https://doi.org/10.1134/S0006297921070099

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