Abstract
Purpose
Caloric restriction (CR) and Roux-en-Y Gastric Bypass (RYGB) are considered effective means of body weight control, but the mechanism by which CR and RYGB protect against high-fat diet (HFD)-induced obesity remains elusive. The browning of white adipose tissue (WAT) is a potential approach to combat obesity. Here we assess whether browning of WAT is involved in CR- and RYGB-treatment.
Methods
The average size of adipocytes was determined by histological analysis. Expression of thermogenic genes in both human subjects and mice were measured by quantitative real-time PCR and immunohistochemical staining.
Results
The average size of adipocytes was bigger, while the expression of thermogenic genes such as uncoupling protein 1 (UCP1), nuclear factor erythroid-2 like 1 (NRF1) and PPARγ coactivator-1 α (PGC1α) were lower in the WAT of obese subjects when compared to lean controls. Both CR and RYGB promoted weight and fat loss. Increment of the average adipocytes size and down-regulation of thermogenic genes were significantly reversed by both CR and RYGB in the WAT of obese mice.
Conclusions
Our findings showed that CR and RYGB significantly improved high-fat diet-induced lipid accumulation by promoting the browning of WAT.
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Data availability
All data relevant to the study are included in the article or uploaded as supplemental information.
Code availability
Not applicable.
Abbreviations
- CR:
-
Caloric restriction
- RYGB:
-
Roux-en-Y Gastric Bypass
- WAT:
-
White adipose tissue
- BAT:
-
Brown adipose tissue
- UCP1:
-
Uncoupling protein 1
- NRF1:
-
Nuclear factor erythroid-2 like-1
- PGC1α:
-
PPARγ coactivator-1 α
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Funding
This work was supported by grants from the National Natural Science Foundation of China (81770794, 314010010), the Fundamental Research Funds for the Central Universities (21620423).
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GX designed research; DH, ZZ, RL and JH performed research; GX, ZD analyzed data; GX, ZD, DH and ZZ wrote and edited the paper. All authors contributed to the discussion and revised the article and all approved the final versions of the manuscript. GX is responsible for the integrity of the work as a whole.
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Participation in this study was voluntary and written informed consent was obtained from each participant. The guidelines of the Declaration of Helsinki of the World Medical Association were followed. All protocols were approved by the Research Ethics Committee of the First Affiliated Hospital of Jinan University.
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Animals used in this study were handled in accordance with the Guide for the Care and Use of Laboratory Animals published by the National Institutes of Health (NIH Publications No. 8023, revised 1978). All animal protocols were approved by the Animal Care and Use Committee of Jinan University.
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Huang, D., Zhang, Z., Dong, Z. et al. Caloric restriction and Roux-en-Y Gastric Bypass promote white adipose tissue browning in mice. J Endocrinol Invest 45, 139–148 (2022). https://doi.org/10.1007/s40618-021-01626-0
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DOI: https://doi.org/10.1007/s40618-021-01626-0