Abstract
In this study, the mechanism that vitamin C (VC) regulates the production of reactive oxygen species (ROS) through Wnt10b signaling was investigated in the gill of zebrafish (Danio rerio). The results showed that 0.5 and 1.0 g/kg VC diets induced the gene expression of Wnt10b, β-catenin, SOD, CAT, and GSH-PX in gill. In addition, VC decreased the levels of H2O2, O2·− and ·OH, whereas the activities of SOD, CAT, and GSH-PX were increased by VC in the gill of zebrafish. To evaluate the role of Wnt10b in regulating oxidative stress, Wnt10b RNA was further interfered and the gene expression and activities of antioxidant enzymes were detected in gill. The result of Wnt10b RNA interference showed that Wnt10b signaling played a key role in regulating the gene expression of SOD, CAT, and GSH-PX. In all, VC may regulate the production of ROS through Wnt10b signaling in the gill of zebrafish (Danio rerio).
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Data availability
The data obtained during and/or analyzed during the current study are available from the corresponding author on reasonable request.
Abbreviations
- ·OH:
-
Hydroxy radical
- CAT:
-
Catalase
- DCFH-DA:
-
2’,7’-Dichlorofluorescein diacetate
- GSH-PX:
-
Glutathione peroxidase
- GSK-3β:
-
Glycogen synthase kinase-3β
- H2O2 :
-
Hydrogen peroxide
- O2 · − :
-
Superoxide anion
- ROI:
-
Region of interest
- ROS:
-
Reactive oxygen species
- SOD:
-
Superoxide dismutase
- VC:
-
Vitamin C
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Funding
This study was supported by Open Project Program of State Key Laboratory of Food Science and Technology, Jiangnan University (SKLF-KF-201905), and Major Innovative Engineering Projects in Shandong Province (2019JZZY020710).
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DW Liu: conceptualization, supervision, methodology; YQ Gu: writing–original draft preparation; HR Yu: investigation, writing–reviewing and editing.
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The experiment was approved by the Animal Care and Use Committee of Shandong University of Technology. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed by the authors.
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Liu, D., Gu, Y. & Yu, H. Vitamin C regulates the production of reactive oxygen species through Wnt10b signaling in the gill of zebrafish. Fish Physiol Biochem 47, 1271–1282 (2021). https://doi.org/10.1007/s10695-021-00982-1
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DOI: https://doi.org/10.1007/s10695-021-00982-1