Abstract
Chemicals used for plant health may have negative impacts on humans, animals and environment, thus antagonistic microorganisms have become popular for the control of plant diseases. Acidovorax citrulli (Ac)-induced melon bacterial fruit blotch (BFB) disease poses serious threats worldwide to the production of cucurbits, especially melon (Cucumis melo) and watermelon (Citrullus lanatus). In this study, effects of 14 different antagonistic bacteria isolated from the phyllosphere of melon and watermelon were tested against BFB through seed treatments under growth chamber conditions and through foliage sprays under greenhouse conditions. When seeds were treated with antagonistic bacteria, seven of them reduced disease incidence by over 50% and disease severity by about 70%. Then, these seven antagonists were sprayed on melon leaves twice (1 and 15 days after transplanting) under greenhouse conditions and disease development was monitored weekly for five weeks. As compared to the control, Pseudomonas oryzihabitans Antg-12, isolated from watermelon leaf, reduced both disease severity (55.85%) and the area under the disease curve (AUDPC) (66.85%), and increased yield by about 41%. In the present study it was shown that certain bacterial biocontrol agents can substantially reduce disease and, when added into integrated management of BLB, may well provide very useful solutions to disease control. To further prove this, studies using the antagonists under open field conditions are planned.
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References
Adhikari M, Yadav DR, Kim SW, Um YH, Kim HS, Lee SC, Song JY, Kim HG, Lee YS (2017) Biological control of bacterial fruit blotch of watermelon pathogen (Acidovorax citrulli) with rhizosphere associated bacteria. Plant Pathol J 33:170–183
Amadi JE, Adebola MO, Eze CS (2009) Isolation and identification of bacterial blotch organism from watermelon (Citrullus lanatus (Thunb.) Matsum. and Nakai). Afr J Agric Res 4:1291–1294
Araujo DV, Mariano RLR, Michereff SJ (2005) Metodos de inoculaçao de Acidovorax avenae subsp. citrulli em melao. Summa Phytopathol 31:66–70
Bahar O, Kritzman G, Burdman S (2009) Bacterial fruit blotch of melon: screens for disease tolerance and role of seed transmission in pathogenicity. Eur J Plant Pathol 123:71–83
Bastas KK (2013) Vegetable bacterial diseases in Turkey. Eur J Plant Pathol Sci Biotechnol (Special Issue 1), 14–24
Belimov AA, Dodd IC, Safronova VI, Shaposhnikov AI, Azarova TS, Makarova NM, Davies WJ, Tikhonovich IA (2015) Rhizobacteria that produce auxins and contain 1-amino-cyclopropane-1-carboxylic acid deaminase decrease amino acid concentrations in the rhizosphere and improve growth and yield of well-watered and water-limited potato (Solanum tuberosum). Ann Appl Biol 167:11–25
Cantabella D, Dolcet-Sanjuan R, Casanovas M, Solsona C, Torres R, Teixido N (2020) Inoculation of in vitro cultures with rhizosphere microorganisms improve plant development and acclimatization during immature embryo rescue in nectarine and pear breeding programs. Sci Hortic 273
Carbo A, Torres R, Usall J, Ballesta J, Teixido N (2020) Biocontrol potential of Ampelomyces quisqualis strain CPA-9 against powdery mildew: Conidia production in liquid medium and efficacy on zucchini leaves. Sci Hortic 267:109337. https://doi.org/10.1016/j.scienta.2020.109337
Choi O, Lee Y, Kang B, Bae J, Kim S, Kim J (2019) Aversion center blackening of muskmelon fruit caused by Pseudomonas oryzihabitans, an opportunistic pathogen of humans and warm-blooded animals. Int J Food Microbiol 291:1–4
Chun-Hao J, Fang W, Zhen-Yun Y, Ping X, Hong-Jiao K, Hong-Wei L, Yi-Yang Y, Jian-Hua G (2015) Study on screening and antagonistic mechanisms of Bacillus amyloliquefaciens 54 against bacterial fruit blotch (BFB) caused by Acidovorax avenae subsp. citrulli. Microbiol Res 170:95–104
Fan H, Zhang Z, Li Y, Zhang X, Duan Y, Wang Q (2017) Biocontrol of bacterial fruit blotch by Bacillus subtilis 9407 via surfactin-mediated antibacterial activity and colonization. Front Microbiol 8:1973. https://doi.org/10.3389/fmicb.2017.01973
Food and Agriculture Organization [FAO] (2018 onwards) FAOSTAT. http://www.fao.org/faostat/en/#data/QC. [24 April 2020]
Fessehaie A, Walcott RR (2005) Biological control to protect watermelon blossoms and seed from infection by Acidovorax avenae subsp. citrulli. Phytopathology 95:413–419
Gafni A, Calderon CE, Harris R, Buxdorf K, Dafa-Berger A, Zeilinger-Reichert E, Levy M (2015) Biological control of the cucurbit powdery mildew pathogen Podosphaera xanthii by means of the epiphytic fungus Pseudozyma aphidis and parasitism as a mode of action. Front Plant Sci 6:132. https://doi.org/10.3389/fpls.2015.00132
González V, Armijos E, Garcés-Claver A (2020) Fungal endophytes as biocontrol agents against the main soil-borne diseases of melon and watermelon in Spain. Agronomy 10:820. https://doi.org/10.3390agronomy10060820
Hopkins DL, Thompson CM (2002) Seed transmission of Acidovorax avenae subsp. citrulli in cucurbits. HortScience 37:924–926
Horuz S, Aysan Y (2012) Outbreak of bacterial fruit blotch disease on melon and watermelon in Cukurova Region, Turkey. 10th EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae, Antalya (Turkey). 763–766
Horuz S, Cetinkaya-Yildiz R, Mirik M, Aysan Y (2014) Occurrence, isolation, and identification of Acidovorax citrulli from melon in Turkey. Plant Prot Sci 50:179–183
Horuz S, Aysan Y (2018) Biological control of watermelon seedling blight caused by Acidovorax citrulli using antagonistic bacteria from the genera Curtobacterium, Microbacterium and Pseudomonas. Plant Prot Sci 54:138–146
Hou Y, Zhang Y, Yu L, Ding X, Liu L, Wang L, Huang S (2020) First report of Pseudomonas oryzihabitans causing rice panicle blight and grain discoloration in China. Plant Dis 104(11):3055
Irizarry I, White JF (2017) Application of bacteria from non-cultivated plants to promote growth, alter root architecture and alleviate salt stress of cotton. J Appl Microbiol 122:1110–1120
Johnson KL, Minsavage GV, Le T, Jones JB, Walcott RR (2011) Efficacy of a nonpathogenic Acidovorax citrulli strain as a biocontrol seed treatment for bacterial fruit blotch of cucurbits. Plant Dis 95:697–704
Lelliott RA, Stead DE (1987) Methods for the Diagnosis of Bacterial Diseases of Plants. Blackwell Scientific Publications, England, Oxford, p 216
McKinney HH (1923) Influence of soil temperature and moisture on infection of wheat seedlings by Helminthosporium sativum. J Agric Res 26:195–218
Melo EA, de Mariano RL, Laranjeira D, Santos AD, Gusmao LO, Souza EB (2015) Efficacy of yeast in the biocontrol of bacterial fruit blotch in melon plants. Tropical Plant Pathology 40:56–64
O’Brien PA (2017) Biological control of plant diseases. Australas Plant Pathol 46:293–304
Panagopoulos GN, Megaloikonomos PD, Liontos M, Giannitsioti E, Drogari-Apiranthitou Miranda Mavrogenis AF, Kontogeorgakos V (2016) Pseudomonas oryzihabitans infected total hip arthroplasty. J Bone Jt Infect 1:54–58
Punja ZK, Tirajoh A, Collyer D, Ni L (2019) Efficacy of Bacillus subtilis strain QST 713 (Rhapsody) against four major diseases of greenhouse cucumbers. Crop Prot 124:104845. https://doi.org/10.1016/j.cropro.2019.104845
Rahimi-Midani A, Choi T-J (2020) Transport of phage in melon plants and ınhibition of progression of bacterial fruit blotch. Viruses 12:477. https://doi.org/10.3390v12040477
Raupach GS, Kloepper JW (1998) Mixtures of plant growth-promoting rhizobacteria enhance biological control of multiple cucumber pathogens. Phytopathology 88:1158–1164
Roberts DC, Fleischer SJ, Sakamoto JM, Rasgon JL (2018) Potential biological control of Erwinia tracheiphila by internal alimentary canal interactions in Acalymma vittatum with Pseudomonas fluorescens. J Appl Microbiol 125:1137–1146
Samaliev H, Andreoglou F, Elawad S, Hague N, Gowen S (2000) The nematicidal effects of the bacteria Pseudomonas oryzihabitans and Xenorhabdus nematophilus on the root-knot nematode Meloidogyne javanica. Nematology 2(5):507–514
Shanner G, Finney RE (1977) The effect of nitrogen fertilization on the expression of slow mildewing resistance in Knox wheat. Phytopathology 67:1051–1056
Tymon LS, Morgan P, Gundersen B, Inglis DA (2020) Potential of endophytic fungi collected from Cucurbita pepo roots grown under three different agricultural mulches as antagonistic endophytes to Verticillium dahliae in western Washington. Microbiol Res 240:126535. https://doi.org/10.1016/j.micres.2020.126535
Vagelas IK, Pembroke, BG, Simon R, Davies KG (2007) The control of root-knot nematodes (Meloidogyne spp.) by Pseudomonas oryzihabitans and its immunological detection on tomato roots. Nematology 9(3):363–370
Walcott RR (2008) Integrated pest management of bacterial fruit blotch of cucurbits. In: Integrated management of diseases caused by fungi, phytoplasma and bacteria. (Editors: Ciancio, A. and Mukerji, K.G.). Springer Book, p:188
Wang X, Li G, Jiang D, Huang H-C (2009) Screening of plant epiphytic yeasts for biocontrol of bacterial fruit blotch (Acidovorax avenae subsp. citrulli) of hami melon. Biol Control 50:164–171
Acknowledgements
The author is thankful to Dr. Zeki Gokalp (Native English speaker) for his critical reading of the manuscript and Erciyes University Scientific Research Projects Unit (Grant Agreement No FBA-2020-10468) for financial assistance.
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Research grants from Erciyes University (Grant number FBA-2020–10468).
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Horuz, S. Pseudomonas oryzihabitans: a potential bacterial antagonist for the management of bacterial fruit blotch (Acidovorax citrulli) of cucurbits. J Plant Pathol 103, 751–758 (2021). https://doi.org/10.1007/s42161-021-00893-3
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DOI: https://doi.org/10.1007/s42161-021-00893-3