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Mapping and validation of quantitative trait loci that confer resistance to rice black-streaked dwarf virus disease in rice (Oryza sativa)

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Abstract

Rice black-streaked dwarf virus (RBSDV) disease is one of the most destructive viral diseases that threatens rice production in China. Breeding of resistant cultivars through multi-gene pyramiding is considered to be an effective way to control the disease, but few resistance genes have been characterized to date. In the present study, we identified T1012, a BC2F6 line from a cross of the japonica variety ‘Wuyujing3’ (recipient) and the indica variety ‘Dular’ (donor), that had improved resistance to RBSDV disease in a field test, and 140 backcross inbred lines (BILs) derived from a cross between T1012 and ‘Wuyujing3’ were developed using marker-assisted selection. Genetic analysis showed that the resistance of T1012 to RBSDV disease was controlled by quantitative trait loci (QTLs). Two QTLs for RBSDV disease resistance located on chromosomes 1 and 4, qRBSDV-1 and qRBSDV-4, were identified, and qRBSDV-4 was repeatedly detected in two environments. Compared to ‘Wuyujing3’, the line containing only the substitution segment covering qRBSDV-4 exhibited significantly decreased disease incidence, indicating that qRBSDV-4 is a reliable resistance QTL with a high breeding value. Furthermore, two linked QTLs, qRBSDV-4-1 and qRBSDV-4-2, were identified within the interval containing qRBSDV-4. The QTLs identified here will provide a useful resource for breeding RBSDV-resistant rice cultivars through marker-assisted selection and establish a foundation for the cloning of RBSDV disease resistance genes.

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Data availability

All data supporting the conclusions of this article are provided within the article (and the Additional files).

Abbreviations

QTL:

Quantitative trait loci

BIL:

Backcross inbred line

MAS:

Marker-assisted selection

RBSDV:

Rice black-streaked dwarf virus

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Funding

This study was financially supported by the National Major Project for Developing New GM Crops (2016ZX08001002-003); the National Natural Science Foundation of China (Grant No. 32001517); the Natural Science Foundation of Jiangsu Province (Grant No. BK20200927); the Government of Jiangsu Province (BE2018357, PAPD, YCSL201904 and PL202004).

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Author notes

  1. Honggen Zhang, Ruixuan Wang and Zuopeng Xu contributed equally to this paper.

Authors and Affiliations

  1. Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Agricultural College of Yangzhou University, Yangzhou, 225009, China

    Honggen Zhang, Ruixuan Wang, Zuopeng Xu, Zhiai Chen, Qiaoquan Liu, Minghong Gu & Shuzhu Tang

  2. Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009, China

    Honggen Zhang, Jiangning Liu, Qiaoquan Liu, Minghong Gu & Shuzhu Tang

  3. Changshu Institute of Agricultural Science, Changshu, 215500, China

    Guofang Lan

Authors
  1. Honggen Zhang
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  2. Ruixuan Wang
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  3. Zuopeng Xu
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  4. Jiangning Liu
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  7. Qiaoquan Liu
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Contributions

HZ conducted the data analysis and drafted the manuscript. RW, ZX, and ZC performed the phenotypic evaluations and data analysis. JL and ZC participated in the construction of the BIL population. QL and MG participated in the design of the study. HZ and ST designed this study and revised the manuscript. All of the authors have read and approved the final manuscript.

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Correspondence to Honggen Zhang or Shuzhu Tang.

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Zhang, H., Wang, R., Xu, Z. et al. Mapping and validation of quantitative trait loci that confer resistance to rice black-streaked dwarf virus disease in rice (Oryza sativa). Euphytica 217, 156 (2021). https://doi.org/10.1007/s10681-021-02890-0

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