Abstract
Considering the constant exposure of fish to copper oxide nanoparticles (CuO-NPs) and copper sulphate (CuSO4) in natural aquatic environments as a result of increased usage of these chemicals in industry, medicine and aquaculture/agriculture over the past few decenniums, the current investigation aimed to reveal their comparative hemotoxic effects on freshwater fish Oreochromis niloticus by measuring hematological and blood oxidative stress biomarkers and Cu levels. Fish were exposed to 0.05 mg/L CuO-NPs and CuSO4 for 4 and 21 days. Both copper forms decreased erythrocyte, hematocrit, and hemoglobin values, and superoxide dismutase and catalase activities while they elevated glucose-6-phosphate dehydrogenase activity, and glutathione, malondialdehyde and Cu levels. Leukocyte levels and glutathione peroxidase activity did not show any significant change. In a conclusion, the current research demonstrates that CuO-NPs and CuSO4 for O. niloticus have similar hemotoxic effects, however, CuO-NPs are slightly more toxic than CuSO4 regarding hematological changes and oxidative stress observed.
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References
Abdel-Khalek AA, Badran SR, Marie MAS (2016) Toxicity evaluation of copper oxide bulk and nanoparticles in Nile tilapia, Oreochromis niloticus, using hematological, bioaccumulation and histological biomarkers. Fish Physiol Biochem 42:1225–1236. https://doi.org/10.1007/s10695-016-0212-8
Abdel-Tawwab M, Mousa MAA (2005) Effect of calcium preexposure on acute copper toxicity to juvenile Nile tilapia, Oreochromis niloticus (L.). Zagazig Vet J 33:80–87
Al-Asgah NS, Abdel-Warith AWA, Younis ESM, Allam HY (2015) Haematological and biochemical parameters and tissue accumulations of cadmium in Oreochromis niloticus exposed to various concentrations of cadmium chloride. Saudi J Biol Sci 22:543–550. https://doi.org/10.1016/j.sjbs.2015.01.002
Alkobaby AI, El-Wahed RKA (2017) The acute toxicity of copper to Nile tilapia (Oreochromis niloticus) fingerlings and its effects on gill and liver histology. J Aquac Res Dev 8:1. https://doi.org/10.4172/2155-9546.1000465
Ayala A, Munoz MF, Arguelles S (2014) Lipid peroxidation: Production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid Med Cell Longev 2014:360438. https://doi.org/10.1155/2014/360438
Beutler E (1984) Red cell metabolism: A manual of biochemical methods, 2nd. edn. Grune and Stration Company, New York
Bhattacharya R, Chatterjee A, Chatterjee S, Saha NC (2021) Oxidative stress in benthic oligochaete worm, Tubifex tubifex induced by sublethal exposure to a cationic surfactant cetylpyridinium chloride and an anionic surfactant sodium dodecyl sulfate. Comp Biochem Physiol 240 C:108906. https://doi.org/10.1016/j.cbpc.2020.108906
Canli EG, Atli G, Canli M (2017) Response of the antioxidant enzymes of the erythrocyte and alterations in the serum biomarkers in rats following oral administration of nanoparticles. Environ Toxicol Pharmacol 50:145–150. https://doi.org/10.1016/j.etap.2017.02.007
Carvalho CS, Fernandes MN (2019) Effects of copper toxicity at different pH and temperatures on the in vitro enzyme activity in blood and liver of fish, Prochilodus lineatus. Mol Biol Rep 46:4933–4942. https://doi.org/10.1007/s11033-019-04944-y
Chatterjee A, Bhattacharya R, Chatterjee S, Saha NC (2021) Acute toxicity of organophosphate pesticide profenofos, pyrethroid pesticide λ cyhalothrin and biopesticide azadirachtin and their sublethal effects on growth and oxidative stress enzymes in benthic oligochaete worm, Tubifex tubifex. Comp Biochem Physiol 242 C:108943. https://doi.org/10.1016/j.cbpc.2020.108943
Dubovskiy IM, Martemyanov VV, Vorontsova YL, Rantala MJ, Gryzanova EV, Glupov VV (2008) Effect of bacterial infection on antioxidant activity and lipid peroxidation in the midgut of Galleria mellonella L. larvae (Lepidoptera, Pyralidae). Comp Biochem Physiol 148 C:1–5. doi:https://doi.org/10.1016/j.cbpc.2008.02.003
Fernandez D, Gomez CG, Babín M (2013) In vitro evaluation of cellular responses induced by ZnO nanoparticles, zinc ions and bulk ZnO in fish cells. Sci Total Environ 452:262–274. https://doi.org/10.1016/j.scitotenv.2013.02.079
Fırat Ö, Bozat RC (2019) Assessment of biochemical and toxic responses induced by titanium dioxide nanoparticles in Nile tilapia Oreochromis niloticus. Hum Ecol Risk Assess 25(6):1438–1447. https://doi.org/10.1080/10807039.2018.1465338
Fırat Ö, Kargın F (2010) Effects of zinc and cadmium on erythrocyte antioxidant systems of a freshwater fish Oreochromis niloticus. J Biochem Mol Toxicol 24:223–229
Fırat Ö, Tutus R (2020) Comparative acute toxicity assessment of organophosphate and avermectin ınsecticides on a freshwater fish Oreochromis niloticus. Bull Environ Contam Toxicol 105:582–587. https://doi.org/10.1007/s00128-020-02990-y
Harabawy ASA, Ibrahim ATA (2014) Sublethal toxicity of carbofuran pesticide on the African catfish Clarias gariepinus (Burchell, 1822): Hematological, biochemical and cytogenetic response. Ecotoxicol Environ Saf 103:61–67. doi:https://doi.org/10.1016/j.ecoenv.2013.09.022
Ighalo JO, Sagboye PA, Umenweke G, Ajala OJ, Omoarukhe FO, Adeyanju CA, Ogunniyi S, Adeniyi AG (2021) CuO nanoparticles (CuO NPs) for water treatment: A review of recent advances. Environ Nanotechnol Monit Manag 15:100443. https://doi.org/10.1016/j.enmm.2021.100443
Ince AT, Kunc S (1988) Mercury in whole blood of persons living in a polluted region of Turkey. J Trace Elem Electrolytes Health Dis 2:97–100
Isani G, Falcioni ML, Barucca G, Sekar D, Andreani G, Carpene E, Falcioni G (2013) Comparative toxicity of CuO nanoparticles and CuSO4 in rainbow trout. Ecotoxicol Environmen Saf 97:40–46. https://doi.org/10.1016/j.ecoenv.2013.07.001
Karadag H, Fırat O, Fırat O (2014) Use of oxidative stress biomarkers in Cyprinus carpio L. for the evaluation of water pollution in Ataturk Dam Lake (Adiyaman, Turkey). Bull Environ Contam Toxicol 92:289–293. https://doi.org/10.1007/s00128-013-1187-0
Lartillot S, Kadziora P, Athios A (1988) Purification and characterization of new fungal catalase. Prepar Biochem 18(3):241–246
Li M, Wang X, Qi C, Li E, Du Z, Qin JG, Chen L (2018) Metabolic response of Nile tilapia (Oreochromis niloticus) to acute and chronic hypoxia stress. Aquaculture 495:187–195. https://doi.org/10.1016/j.aquaculture.2018.05.031
Lind CE, Agyakwah SK, Attipoe FY, Nugent C, Crooijmans RPMA, Toguyeni A (2019) Genetic diversity of Nile tilapia (Oreochromis niloticus) throughout West Africa. Sci Rep 9:16767. https://doi.org/10.1038/s41598-019-53295-y
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurements with the folin phenol reagent. J Biol Chem 193:265–275
Mekkawy IAA, Mahmoud UM, Mohammed RH (2013) Protective effects of tomato paste and vitamin E on atrazine-induced hematological and biochemical characteristics of Clarias gariepinus (Burchell, 1822). Global Adv Res J Environ Sci Toxicol 2:11–21
Ohadi S, Godar A, Madsen J, Al-Khatib K (2021) Response of rice algal assemblage to fertilizer and chemical application: Implications for early algal bloom management. Agronomy 11:542. https://doi.org/10.3390/agronomy11030542
Shaw BJ, Al-Bairuty G, Handy RD (2012) Effects of waterborne copper nanoparticles and copper sulphate on rainbow trout, (Oncorhynchus mykiss): Physiology and accumulation. Aquat Toxicol 116:90–101. https://doi.org/10.1016/j.aquatox.2012.02.032
Sun Y, Oberley LW, Li Y (1988) A simple method for clinical assay of superoxide dismutase. Clin Chem 34:497–500
Tunçsoy M, Duran S, Ay Ö, Cicik B, Erdem C (2017) Effects of Copper oxide nanoparticles on antioxidant enzyme activities and on tissue accumulation of Oreochromis niloticus. Bull Environ Contam Toxicol 99:360–364. doi:https://doi.org/10.1007/s00128-017-2129-z
Wang T, Wen X, Hu Y, Zhang X, Wang D, Yin S (2019) Copper nanoparticles induced oxidation stress, cell apoptosis and immune response in the liver of juvenile Takifugu fasciatus. Fish Shellfish Immunol 84:648–655. https://doi.org/10.1016/j.fsi.2018.10.053
Xylina TG, Prchal JT (2018) Hematology: Basic principles and practice, 7th edn. Elsevier, Philadelphia
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This study was partially funded by the Scientific Research Projects Unit of Adiyaman University (Grant of FEFYL/2018-0002).
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Fırat, Ö., Erol, R. & Fırat, Ö. An Investigation on Freshwater Fish Oreochromis niloticus (Linnaeus, 1758): Assessing Hemotoxic Effects of Different Copper Compounds Used as Nanomaterial or Pesticide. Bull Environ Contam Toxicol 108, 549–554 (2022). https://doi.org/10.1007/s00128-021-03320-6
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DOI: https://doi.org/10.1007/s00128-021-03320-6