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Alternative splicing of medaka bcl6aa and its repression by Prdm1a and Prdm1b

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Abstract

Bcl6 and Prdm1 (Blimp1) are a pair of transcriptional factors that repressing each other in mammals. Prdm1 represses the expression of bcl6 by binding a cis-element of the bcl6 gene in mammals. The homologs of Bcl6 and Prdm1 have been identified in teleost fish. However, whether these two factors regulate each other in the same way in fish like that in mammals is not clear. In this study, the regulation of bcl6aa by Prdm1 was investigated in medaka. The mRNA of bcl6aa has three variants (bcl6aaX1-X3) at the 5′-end by alternative splicing detected by RT-PCR. The three variants can be detected in adult tissues and developing embryos of medaka. Prdm1a and prdm1b are expressed in the tissues and embryos where and when bcl6aa is expressed. The expression of prdm1a was high while the expression of bcl6aa was low, and vice versa, detected in the spleen after stimulation with LPS or polyI:C. In vitro reporter assay indicated that bcl6aa could be directly repressed by both Prdm1a and Prdm1b in a dosage-dependent manner. After mutation of the key base, G, of all predicted binding sites in the core promoter region of bcl6aa, the repression by Prdm1a and/or Prdm1b disappeared. The binding site of Prdm1 in the bcl6aa gene is GAAAA(T/G). These results indicate that both Prdm1a and Prdm1b directly repress the expression of bcl6aa by binding their binding sites where the 5′-G is critical in medaka fish.

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Funding

This work was supported by the Natural Science Foundation of China (Grant No. 31672284 to HZ) and the Open Fund of Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances (No. PTS2020-02 to HZ).

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Author notes

  1. Xiaomei Ke and Runshuai Zhang contributed equally to this work.

Authors and Affiliations

  1. Hubei Key Laboratory of Gene Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, Hubei, China

    Xiaomei Ke, Runshuai Zhang, Qiting Yao, Shi Duan, Wentao Hong, Qingchun Zhou, Xueping Zhong & Haobin Zhao

  2. Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances, Institute of Environment and Health, Jianghan University, Wuhan, 430056, China

    Mengxi Cao

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  1. Xiaomei Ke
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  4. Shi Duan
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Contributions

Qingchun Zhou, Xueping Zhong, and Haobin Zhao: conceptualization and designing the experiment; Xiaomei Ke, Runshuai Zhang, Qiting Yao, and Shi Duan: experimental setup and execution; Wentao Hong and Mengxi Cao: data analysis; Xiaomei Ke, Runshuai Zhang, and Haobin Zhao: manuscript writing.

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Correspondence to Haobin Zhao.

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This study was carried out in strict accordance with recommendations in the Regulation for the Management of Laboratory Animals of the Ministry of Science and Technology of China. The animal protocol for this study was approved by the Animal Care and Use Committee of Hubei Province in China [No. SYXK(E)2015–0012]. None of the fish suffered starvation, trauma, or electrical shock, and all the fish were completely anesthetized before tissue sampling.

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Ke, X., Zhang, R., Yao, Q. et al. Alternative splicing of medaka bcl6aa and its repression by Prdm1a and Prdm1b. Fish Physiol Biochem 47, 1229–1242 (2021). https://doi.org/10.1007/s10695-021-00980-3

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