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Supplementation with Phaseolus vulgaris Leaves Improves Metabolic Alterations Induced by High-Fat/Fructose Diet in Rats Under Time-Restricted Feeding

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Abstract

Time-restricted feeding and food enriched in polyphenols are strategies to prevent or reduce metabolic disorders. Bean leaves (Phaseolus vulgaris L.) are a recognized source of polyphenolic compounds, whose effects on metabolic pathways are not well studied. We evaluated the combined effects of dietary supplementation with Phaseolus vulgaris leaves (10% w/w) (BL) and a 7-h daytime-restricted feeding protocol (RF) under a hypercaloric diet (high fat + high fructose) (HFFD) on the metabolic parameters related to glucose and lipid handling. Adult male Wistar rats were treated for 8 weeks with standard and HFFD diets with or without BL. The results showed that RF improved metabolic alterations induced by HFFD (e.g., hepatic steatosis, increased triacylglycerols, and serum lipoproteins). Supplementation with BL significantly enhanced this effect and downregulated the mRNA expression of carbohydrate and lipid metabolism genes in the liver. These results indicate that dietary supplementation with BL enhances the benefits elicited by RF.

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Abbreviations

Acc:

Acetyl-CoA carboxylase

BL:

Bean leaves

Fas:

Fatty acid synthase

G6pase:

Glucose-6-phosphatase

G6pd:

Glucose-6-phosphate dehydrogenase

Gk:

Glucokinase

HDL:

High-density lipoprotein

HFFD:

High fat + high fructose diet

HOMA-IR:

Homeostatic Model Assessment for Insulin Resistance

LDL:

Low-density lipoprotein

Pepck:

Phosphoenolpyruvate carboxykinase

Pklr:

Pyruvate kinase

RF:

Restricted feeding

Scd1:

Stearoyl-CoA desaturase-1

SD:

Standard diet

TAG:

Triacylglycerides

VLDL:

Very-low-density lipoprotein

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Acknowledgements

We thank Jéssica González Norris for assistance with the English version of this manuscript, and Dr. Christian Molina Aguilar, Dr. Aracely Anaya-Loyola, Nut. Sonia Hernández-Nájera, Nut. Fernando López-Barrera, Lilia Ruíz-Garduño, Biol. Josué López-Martínez, Ing. Nydia Hernández-Ríos, and Nut. Adriana Becerril-Campos for their technical support.

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Author notes

  1. G. Ramírez-Venegas and D.L. De Ita-Pérez are contributed equally to this work.

Authors and Affiliations

  1. CONACYT, Av. Insurgentes Sur 1582, Col. Crédito Constructor, Alcaldía Benito Juárez, 03940, Ciudad de México, México

    D. Luna-Moreno

  2. Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Av. de las Ciencias S/N., Juriquilla, 76230, Querétaro, México

    G. Ramírez-Venegas, T. García-Gasca, M. Ahumada-Solórzano, X. Zambrano-Estrada & D. Luna-Moreno

  3. Departamento de Neurobiología Celular y Molecula, Instituto de Neurobiología, Universidad Nacional Autónoma de México, Campus UNAM-Juriquilla, Querétaro, 76230, QRO, México

    DL. De Ita-Pérez, M. Díaz-Muñoz, I. Méndez & O. Vázquez-Martínez

  4. Centro de Investigación Regional del Centro, Univ. de Biotecnología, Campo Experimental Bajío, INIFAP Km 6, Carr. Celaya-San Miguel Allende, 112, Celaya, Guanajuato, Mexico

    H. Guzmán-Maldonado

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  1. G. Ramírez-Venegas
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  2. DL. De Ita-Pérez
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Correspondence to D. Luna-Moreno.

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Ramírez-Venegas, G., De Ita-Pérez, D., Díaz-Muñoz, M. et al. Supplementation with Phaseolus vulgaris Leaves Improves Metabolic Alterations Induced by High-Fat/Fructose Diet in Rats Under Time-Restricted Feeding. Plant Foods Hum Nutr 76, 297–303 (2021). https://doi.org/10.1007/s11130-021-00904-9

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