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Novel Protein Hydrocolloids Constructed by Hydrophobic Rice Proteins and Walnut Proteins as Loading Platforms for Nutraceutical Models

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Abstract

Low water solubility strictly limits the application potential of such plant-derived proteins as rice proteins (RPs) and walnut proteins (WPs), albeit their nutritional and health-related properties. In this study, by simply dissolving RPs and WPs at pH 12 prior to neutralization, we successfully prepared nanoscale hydrocolloidal composites with shared internal molecular arrangements, boosting the solubility of RPs to over 80% (w/v) while completely solubilizing WPs. Atomic force microscopy and transmission electron microscopy showed that the two polypeptide chains were packed into homogeneous particles with a diameter ranging from 50 to 100 nm. Varying the mass ratio of RPs/WPs enabled the flexible or rigid chain configuration, which was confirmed by static and dynamic light scattering. The results from zeta-potential and surface hydrophobicity demonstrated that the burial of hydrophobic groups and the exposure of charged moieties stipulated the aqueous stability of the protein composites. The apigenin encapsulated in protein composites showed preferable aqueous solubility. Moreover, the improvement of bioaccessibility of apigenin was proved by in vitro simulated digestion experiment. This study provided a new route for utilizing underdeveloped protein resources, especially those with hydrophobic attributes, and potentially expanding the applications of these proteins in the fields of food and related areas.

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Acknowledgements

This work was supported by the the National Natural Science Foundation of China (Grant NO. 31901602 & NO. 31778198), Jiangsu Key Laboratory of Advanced Food Manufacturing Equipment and Technology (NO. FMZ202005), and the National first-class discipline program of Food Science and Technology, China (NO. JUFSTR20180203).

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Authors and Affiliations

  1. Jiangsu Key Laboratory of Advanced Food Manufacturing Equipment and Technology, Jiangnan University, Wuxi, 214122, China

    Fangsi Li, Tao Wang, Wei Feng, Ren Wang & Zhengxing Chen

  2. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, China

    Fangsi Li, Tao Wang, Wei Feng, Ren Wang & Zhengxing Chen

  3. Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China

    Fangsi Li, Tao Wang, Wei Feng, Ren Wang & Zhengxing Chen

  4. School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China

    Fangsi Li, Tao Wang, Wei Feng, Ren Wang & Zhengxing Chen

  5. Jiangsu Provincial Research Centre for Bioactive Product Processing Technology, Jiangnan University, Wuxi, 214122, China

    Fangsi Li, Tao Wang, Wei Feng, Ren Wang & Zhengxing Chen

  6. Anhui Guotai Biotechnology CO., LTD, Xuancheng, 242100, China

    Dalong Yi

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  1. Fangsi Li
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  2. Tao Wang
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  3. Wei Feng
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  4. Ren Wang
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  5. Zhengxing Chen
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  6. Dalong Yi
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Contributions

Fangsi Li: Conceptualization, methodology, investigation, writing-original draft preparation, writing-reviewing and editing, visualization. Tao Wang: Resources, writing-reviewing and editing, supervision. Wei Feng: Visualization, writing-reviewing and editing. Ren Wang: Resources, validation. Zhengxing Chen: Project administration, supervision. Dalong Yi: Conceptualization, funding acquisition.

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Correspondence to Zhengxing Chen or Dalong Yi.

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Highlights

• Hydrocolloids were assembled by hydrophobic rice proteins and walnut proteins.

• The protein composites were designed as the delivery platform for apigenin.

• The in vitro bioaccessibility of apigenin was improved by the protein nanovehicle.

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Li, F., Wang, T., Feng, W. et al. Novel Protein Hydrocolloids Constructed by Hydrophobic Rice Proteins and Walnut Proteins as Loading Platforms for Nutraceutical Models. Food Biophysics 16, 427–439 (2021). https://doi.org/10.1007/s11483-021-09680-0

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  • DOI: https://doi.org/10.1007/s11483-021-09680-0

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