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Adaptive Evolution of Lactococcus Lactis to Thermal and Oxidative Stress Increases Biomass and Nisin Production

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Abstract

High values of agitation and temperature lead to stressful conditions in the fermentations of Lactococcus lactis due to its aero-tolerant and mesophilic nature. Here, the adaptive laboratory evolution (ALE) technique was applied to increase biomass and nisin production yields by enhancing L. lactis subsp. lactis robustness at higher growth temperature and aeration rates. In two separate ALE experiments, after 162 serial transfers, optimum agitation and growth temperature of L. lactis were shifted from 40 rpm and 30 °C to 200 rpm and 37 °C, respectively. Oxidative and acid resistance were enhanced in the evolved strain. Whole-genome sequencing revealed the emergence of five single-nucleotide polymorphisms in the genome of the evolved strain in jag, DnaB, ArgR, cation transporter genes, and one putative protein. The evolved strain of L. lactis in this study has more industrial desirable features and improved nisin production capability and can act more efficiently in nisin production in stressful conditions.

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The genomic sequence data were deposited and available in GenBank under the accession number SAMN12990279. The bacterial strains were deposited in the University of Tehran Microorganisms Collection.

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Authors and Affiliations

  1. Department of Microbial Biotechnology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran

    Reyhaneh Papiran & Javad Hamedi

  2. Microbial Technology and Products (MTP) Research Center, University of Tehran, Tehran, Iran

    Reyhaneh Papiran & Javad Hamedi

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  1. Reyhaneh Papiran
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RP has done the experiments and wrote the manuscript, and JH designed and supervised the experiments and edited the manuscript.

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Correspondence to Javad Hamedi.

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Papiran, R., Hamedi, J. Adaptive Evolution of Lactococcus Lactis to Thermal and Oxidative Stress Increases Biomass and Nisin Production. Appl Biochem Biotechnol 193, 3425–3441 (2021). https://doi.org/10.1007/s12010-021-03609-6

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