Abstract
The combined effect of the irradiation with a proton pencil scanning beam (PBS) at a total dose of 80 Gy and neutron radiation at a dose of 5 Gy on the growth of solid Ehrlich ascites carcinoma (EAC) and the remote effects in tumor-bearing mice was studied. Combined irradiation of mice with neutrons before and after irradiation with PBS, as well as irradiation only with PBS, effectively suppressed the growth of solid EAC within 1 month. In terms of the frequency and severity of radiation-induced skin reactions of mice observed 15–40 days after therapy, neutron irradiation after the irradiation with PBS showed better values of these parameters as compared to only PBS; however, exposure to neutrons before PBS was more damaging as compared to the other two options. It was also shown that the tumor relapse rate in the groups of animals with combined irradiation was higher, and the total lifespan was lower than the group of mice irradiated with PBS alone.
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ACKNOWLEDGMENTS
We are grateful to A.A. Lychagin, S.N. Koryakin, and T.S. Khozyasheva, stuff of the Department of Radiation Biophysics of the National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, Obninsk, Russia, A. Tsyb Medical Radiological Research Center—branch) for help in organizing and conducting the experiment on neutron irradiation using the equipment of the Center.
Funding
The study on the investigation of the combined effect of neutron and proton radiation on the growth of the solid form of Ehrlich ascites carcinoma and its long-term consequences in mice was performed on the basis of the Physical Technical Center of the Lebedev Physical Institute and the Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences in the framework of basic research on topics of state assignment with partial financial support of the R&D agreement no. 01/16 between the Physical Technical Center of the Lebedev Physical Institute and the Institute of Theoretical and Experimental Biophysics.
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Statement on the welfare of animals. All applicable international, national, and institutional guidelines for the care and use of animals were followed. The study was carried out in accordance with the requirements of the Federation of European Scientific Associations on the maintenance and use of laboratory animals in scientific research (Federation of European Laboratory Animal Science Association, FELASA).
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Translated by M. Batrukova
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Balakin, V.E., Rozanova, O.M., Smirnova, E.N. et al. Combined Effect of Neutron and Proton Radiations on the Growth of Solid Ehrlich Ascites Carcinoma and Remote Effects in Mice. Dokl Biochem Biophys 498, 159–164 (2021). https://doi.org/10.1134/S1607672921030017
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DOI: https://doi.org/10.1134/S1607672921030017