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Androgenic Sensitivities and Ovarian Gene Expression Profiles Prior to Treatment in Japanese Eel (Anguilla japonica)

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Abstract

Androgens stimulate ovarian development in eels. Our previous report indicated a correlation between the initial (debut) ovarian status (determined by kernel density estimation (KDE), presented as a probability density of oocyte size) and the consequence of 17MT treatment (change in ovary). The initial ovarian status appeared to be an important factor influencing ovarian androgenic sensitivity. We postulated that the sensitivities of initial ovaries are correlated with their gene expression profiles. Japanese eels underwent operation to sample the initial ovarian tissues, and the samples were stored in liquid nitrogen. Using high-throughput next-generation sequencing (NGS) technology, ovarian transcriptomic data were mined and analyzed based on functional gene classification with cutoff-based differentially expressed genes (DEGs); the ovarian status was transformed into gene expression profiles globally or was represented by a set of gene list. Our results also implied that the initial ovary might be an important factor influencing the outcomes of 17MT treatments, and the genes related with neuronal activities or neurogenesis seemed to play an essential role in the positive effect.

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Acknowledgements

We would like to thank Miss Pei-Syuan Huang and Mr. Xuan-Hao Wu (Department of Life Science, NUK, Taiwan) for helping with the wet bench manipulations; the encouragement from Mr. Yung-Lin Huang and Mr. Yi-Sen Lai was appreciated sincerely.

Funding

This study was partly funded by the Ministry of Science and Technology (MOST107-2321-B002-057), Taiwan.

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Authors and Affiliations

  1. Department of Life Science, National University of Kaohsiung, No. 700 Kaohsiung University Road, Nan Tzu Dist, 811, Kaohsiung, Taiwan

    Yung-Sen Huang

  2. Institute of Information Science, Academia Sinica, No. 128 Academia Road, Section 2, Nankang Dist., 115, Taipei, Taiwan

    Wen-Chih Cheng & Chung-Yen Lin

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  1. Yung-Sen Huang
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  2. Wen-Chih Cheng
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Yung-Sen Huang: corresponding author Wen-Chih Cheng: produce all figures and tables. Chung-Yen Lin: provide opinion on data analysis and results discussion.

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Correspondence to Yung-Sen Huang.

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Huang, YS., Cheng, WC. & Lin, CY. Androgenic Sensitivities and Ovarian Gene Expression Profiles Prior to Treatment in Japanese Eel (Anguilla japonica). Mar Biotechnol 23, 430–444 (2021). https://doi.org/10.1007/s10126-021-10035-6

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