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A novel ampelovirus associated with mealybug wilt of pineapple (Ananas comosus)

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Abstract

Mealybug wilt of pineapple (MWP) is the most important and complex viral disease affecting pineapple worldwide. High-throughput sequencing was conducted to characterize a new virus identified only in symptomatic pineapple plants and tentatively named pineapple mealybug wilt-associated virus 6 (PMWaV-6). Data analyses revealed a genome of 17,854 nucleotides with an organization resembling members of the genus Ampelovirus, family Closteroviridae. Encoded proteins shared sequence identity with the corresponding proteins of grapevine leafroll-associated virus 3, blackberry vein banding-associated virus, and PMWaV-2. The present study reports the discovery of PMWaV-6, a putative and distinct new member of the genus Ampelovirus, subgroup I, its potential involvement in MWP, and the development of PMWaV-6-specific RT-PCR assays to detect and monitor this virus in field samples.

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Acknowledgements

The research was supported in part by Grants from the United State Department of Agriculture National Institute of Food and Agriculture, Hatch HAW09025-H (1001478), and the United State Department of Agriculture -Agricultural Research Service (58-5320-4-012).

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Contributions

JH, ALS and AOV conceived and designed the experiments. ALS performed the experiments. ALS and AOV performed the analysis and wrote the manuscript. JH and MM supervised the study. All authors edited and reviewed the manuscript.

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Correspondence to John Hu.

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The authors declare that they have no conflicts of interest.

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This study did not include experiments with human or animal participants performed by any of the authors.

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Edited by Seung-Kook Choi.

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Larrea-Sarmiento, A., Olmedo-Velarde, A., Wang, X. et al. A novel ampelovirus associated with mealybug wilt of pineapple (Ananas comosus). Virus Genes 57, 464–468 (2021). https://doi.org/10.1007/s11262-021-01852-x

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  • DOI: https://doi.org/10.1007/s11262-021-01852-x

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