Abstract
The present study involved incorporation of two major QTLs for pre-harvest sprouting tolerance (PHST) in an Indian wheat cultivar named Lok1, which happens to be PHS susceptible. For transfer of two QTLs, two independent programmes with two different donors (AUS1408, CN19055) were utilized. The recipient cv. Lok1 was crossed with each of the two donors, followed by a number of backcrosses. Each backcross progeny was subjected to foreground and background selections. KASP assay was also used for confirming the presence of PHST QTL. In one case, PHST QTL was later also pyramided with a gene for high grain protein content (Gpc-B1) and a gene for leaf rust resistance (Lr24). The MAS derived lines were screened for PHS using simulated rain chambers leading to selection of 10 PHST lines. Four of these advanced lines carried all the three QTL/genes and exhibited high level of PHST (PHS score 2–3) associated with significant improvement in GPC and resistance against leaf rust.
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Acknowledgements
PKG was awarded Hony Scientist position and HSB was awarded Senior Scientist position both from Indian National Science Academy (INSA). Head, Department of Genetics and Plant Breeding, CCS University, Meerut, provided the necessary infrastructure.
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Funds for this study were received from DBT, Govt of India (File No. DBT/PR11695/AGR/02/643/2008).
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PKG and HSB designed the experiment. TG carried out phenotyping and data analysis and prepared first draft of the MS. PA, ST, VJ, VG, and SK performed the initial breeding work (including crosses). KK assisted TG in phenotypic data recording. PP carried out rust screening of the pyramided lines. PC provided facilities for KASP assay.
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Gautam, T., Kumar, K., Agarwal, P. et al. Development of white-grained PHS-tolerant wheats with high grain protein and leaf rust resistance. Mol Breeding 41, 42 (2021). https://doi.org/10.1007/s11032-021-01234-z
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DOI: https://doi.org/10.1007/s11032-021-01234-z