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Functional analysis of rice OSCA genes overexpressed in the arabidopsis osca1 mutant due to drought and salt stresses

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Abstract

Drought and salt are two major abiotic stresses that severely impact plant growth and development, as well as crop production. A previous study showed that OsOSCA1.4, one of eleven rice OSCAs (OsOSCAs), complements hyperosmolality-induced [Ca2+]cyt increases (OICIcyt), salt stress-induced [Ca2+]cyt increases (SICIcyt) and the associated growth phenotype in Arabidopsis osca1 (reduced hyperosmolality-induced [Ca2+]cyt increase 1). In this study, Except for OsOSCA2.3 and OsOSCA4.1, we generated independent transgenic lines overexpressing eight other OsOSCAs in the osca1 to explore their functions in osmotic Ca2+ signalling, stomatal movement, leaf water loss, and root growth in response to hyperosmolality and salt stress. Similar to OsOSCA1.4, overexpression of OsOSCA1.1 or OsOSCA2.2 in osca1 complemented OICIcyt and SICIcyt, as well as stomatal closure and root growth in response to hyperosmolality and salt stress treatments, and drought-related leaf water loss. In addition, overexpression of OsOSCA1.2, OsOSCA1.3 or OsOSCA2.1 in osca1 restored OICIcyt and SICIcyt, whereas overexpression of OsOSCA2.5 or OsOSCA3.1 did not. Moreover, osca1 overexpressing these five OsOSCAs exhibited various abiotic stress-associated growth phenotypes. However, overexpression of OsOSCA2.4 did not have any of these effects. These results indicated that multiple members of the OsOSCA family have redundant functions in osmotic sensing and diverse roles in stress adaption.

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Acknowledgements

This work was co-supported by the National Key Basic Research Program of China (973 Program, 2013CB126902), the Beijing Municipal Science and Technology Project (Z121105002812042), and the National Natural Science Foundation of China (grant no. 31070250).

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Authors and Affiliations

  1. Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing, 100875, People’s Republic of China

    Yuanjun Zhai, Zhaohong Wen, Wenqi Fang, Yinxing Wang, Chao Xi, Jin Liu, Heping Zhao, Yingdian Wang & Shengcheng Han

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  1. Yuanjun Zhai
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  2. Zhaohong Wen
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  3. Wenqi Fang
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  4. Yinxing Wang
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  5. Chao Xi
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  6. Jin Liu
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  7. Heping Zhao
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  8. Yingdian Wang
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  9. Shengcheng Han
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Contributions

Y.Z., H. Z., Y-d. W. and S.H. conceived the study and analysed the data. Y.Z., Z.W., W. F., Y-x.W., C. X. and J. L. performed the experiments. Y.Z. drafted the manuscript. Y.Z. and S. H. revised the manuscript.

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Correspondence to Shengcheng Han.

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Zhai, Y., Wen, Z., Fang, W. et al. Functional analysis of rice OSCA genes overexpressed in the arabidopsis osca1 mutant due to drought and salt stresses. Transgenic Res 30, 811–820 (2021). https://doi.org/10.1007/s11248-021-00270-x

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