Abstract
Diet-induced fatty liver is a considerable threaten to fish aquaculture due to the popularity of the high-fat diet (HFD) feeding. Our study aims to investigate the effects of flavanones from Sedum sarmentosum Bunge (FSSB) on the liver function to identify a potential treatment for HFD-induced fatty liver disease. Physiological and pathological indicators were tested in the liver of Nile tilapia (Oreochromis niloticus) and results showed parameters including lipid metabolites, redox parameters, and inflammatory factors could be adequately restored to normal level by addition of 150 mg/kg FSSB to HFD. Proteomics analysis was performed in liver tissues from tilapia with normal diet (ND), HFD, and HFD+FSSB. Totally, 51 upregulated proteins and 77 downregulated proteins were identified in HFD groups and 67 proteins of them were restored after treated with FSSB. Bioinformatics analysis showed that differentially expressed proteins (DEPs) in HFD+FSSB150 group compared with HFD group are mainly enriched in acety-CoA metabolic process, adenosine-triphosphate (ATP) biosynthetic process, lipid metabolic process, and phospholipid metabolic process. The dysregulated proteins were involved in peroxidosome proliferators-activated receptor (PPAR) signaling pathway, fat digestion and absorption, and immune system. The quantitative real-time PCR (qRT-PCR) assay further revealed that the expression of GST, PPARα, PPARγ, and multiple-inflammatory cytokines could be also reversed in HFD group under the treatment of 150 mg/kg FSSB. Our findings demonstrated FSSB is efficient for the treatment of fatty liver disease through regulation of lipid metabolism and antioxidation in Nile tilapia, providing a new treatment of non-alcoholic fatty liver disease (NAFLD) in fish aquaculture.
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Acknowledgements
We thank the State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources (SKLCUSA) for LC-MS/MS analysis.
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The data used to support the findings of this study are available from the corresponding author upon request.
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This work was supported by the Natural Science Foundation of Guangxi Province, Grant/Award Number: No. 2018GXNSFDA281001; the Natural Science Foundation of Guangxi (Grant No. 2016GXNSFAA380233); and the Key Research and Development Projects of Guangxi, Grant/Award Number: No.AB18294011.
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K.H. and Z.T. proposed the idea and designed the experiments. K.Y. performed the proteomic experiment and participated in bioinformatics analysis. X.H. and L.S. prepared the animal materials and established the validation. L.P. and C.M. analyzed the original data. K.Y wrote the original manuscript. K.H revised and finalized the manuscript. All authors have read and approved the manuscript.
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Highlights
We investigated hepatoprotective effects of flavanones from Chinese herb on fatty liver disease in tilapia through the regulation of lipid metabolism, antioxidation, and immunity.
Supplementary materials
Table S1
Main ingredients of FSSB detected by LC-MS/MS. (XLSX 11 kb)
Table S2
Formulation and proximate composition of the experimental diets. (XLSX 9 kb)
Table S3
Scoring criteria of NAS. (XLSX 8 kb)
Table S4
Primer information of qRT-PCR. (XLSX 9 kb)
Table S5
Growth performance of Nile tilapia. (XLSX 10 kb)
Figure S1
DEPs in PPAR signaling pathway between HFD group and ND group. Note: the green color is down-regulated protein expression. (PNG 32 kb)
Figure S2
DEPs in PPAR signaling pathway between HFD+FSSB150 group and HFD group. Note: the red color is up-regulated protein expression. (PNG 32 kb)
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Yu, K., Huang, K., Tang, Z. et al. Metabolism and antioxidation regulation of total flavanones from Sedum sarmentosum Bunge against high-fat diet-induced fatty liver disease in Nile tilapia (Oreochromis niloticus). Fish Physiol Biochem 47, 1149–1164 (2021). https://doi.org/10.1007/s10695-021-00964-3
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DOI: https://doi.org/10.1007/s10695-021-00964-3