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Potato Introgressive Hybridisation Breeding for Bacterial Wilt Resistance Using Solanum commersonii Dun. as Donor: Genetic and Agronomic Characterisation of a Backcross 3 Progeny

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Abstract

Bacterial wilt caused by Ralstonia solanacearum is the main bacterial disease in potato. Solanum commersonii Dun. (cmm; 2n = 2x = 24, 1 EBN) is a native species to southern Brazil, Uruguay and Argentina with desirable traits for introgressive hybridisation breeding into cultivated potato such as resistance to R. solanacearum. In Uruguay, successful crosses between cmm and Solanum tuberosum Group Tuberosum (tbr; 2n = 4x = 48, 4 EBN) have been carried out with this objective, resulting in backcross 1, 2 and 3 progenies. The aim of this study was to characterise one backcross 3 progeny (BC3) using cytogenetic, genetic, morphological and agronomic descriptors. Resistance to R. solanacearum showed transgressive segregation and an association with plant architecture. Fifty-two percent of individuals had chromosome numbers close to cultivated potato with no evidence of preferential loss of cmm chromosomes. All BC3 individuals showed male sterility, probably due to nuclear-cytoplasmic interactions. Although there was wide segregation in morphological traits, most individuals resembled the recurrent tbr parents. A few more backcrosses combined with screening for bacterial wilt resistance may be necessary to allow for further recombination and removal of undesirable traits from cmm. The presence of BC3 individuals with chromosome numbers close to 2n = 48, combining morphological traits from tbr with good levels of resistance, suggests the occurrence of introgression events. This points to S. commersonii as one of the most promising genetic resources for potato breeding from the Southern Atlantic region.

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Acknowledgements

Research was supported by Agencia Nacional de Investigación e Innovación (ANII) and Departamento de Biología Vegetal of Facultad de Agronomía - Universidad de la República (Udelar). We are thankful to Cristina Mazzela, Magdalena Vaio and the staff of Laboratorio de Evolución y Domesticación de Plantas of Facultad de Agonomía - Udelar for the permanent support, patience and valuable suggestions. We wish to thank Pablo Sandro, Matías Antuoni and Julio Sburlatti for their assistance during the development of the experiments. We also wish to thank Gervasio Krismanich, Victoria Dodera, Soraya Silva and Rodrigo Chiruzzo for their assistance with microsatellite markers amplification and genotyping. We are also grateful to Alicia Castillo, Victoria Bonecarrere and the technical staff at INIA and María Ines Siri, Maria Julia Pianzola and the staff at Laboratorio de Microbiología of Facultad de Química - Udelar for kindly providing plant material and for inoculation preparation. We also want to thank Elsa L. Camadro for her useful suggestions.

Funding

This study was funded by the Agencia Nacional de Investigación e Innovación (ANII) grant code FMV_1_2011_ 1_6697 and Departamento de Biología Vegetal of Facultad de Agronomía - Universidad de la República. Mariana Andino was supported by grant ANII POS_NAC_2012_1_8632.

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  1. Facultad de Agronomía, Universidad de la República, Garzón 780, PC 12900, Montevideo, Uruguay

    Mariana Andino, Paola Gaiero, Pablo González-Barrios, Guillermo Galván, Francisco Vilaró & Pablo Speranza

  2. Horticulture Unit, National Institute for Agricultural Research, Ruta 48 km 10, Las Brujas, Uruguay

    Francisco Vilaró

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  1. Mariana Andino
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Mariana Andino, Paola Gaiero and Pablo González-Barrios. Francisco Vilaró produced the hybrids and backcrosses through interspecific crosses. Francisco Vilaró and Guillermo Galván supervised the field trial. Pablo Speranza supervised data analyses and interpretation. The first draft of the manuscript was written by Mariana Andino, Paola Gaiero and Pablo Speranza, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Andino, M., Gaiero, P., González-Barrios, P. et al. Potato Introgressive Hybridisation Breeding for Bacterial Wilt Resistance Using Solanum commersonii Dun. as Donor: Genetic and Agronomic Characterisation of a Backcross 3 Progeny. Potato Res. 65, 119–136 (2022). https://doi.org/10.1007/s11540-021-09512-1

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