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Both overlapping and independent loci underlie seed number per pod and seed weight in Brassica napus by comparative quantitative trait loci analysis

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A Correction to this article was published on 27 August 2021

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Abstract

Seed number per pod (SNPP) and seed weight (SW) are two components of seed yield in rapeseed (Brassica napus). Here, a natural population of rapeseed was employed for genome-wide association analysis for SNPP and SW across multi-years. A total of 101 and 77 SNPs significantly associated with SNPP and SW with the phenotypic variances (R2) ranging from 1.35 to 29.47% and from 0.78 to 34.58%, respectively. And 43 and 33 homologs of known genes from model plants were located in the 65 and 49 haplotype blocks (HBs) for SNPP and SW, respectively. Notably, we found 5 overlapping loci and 3 sets of loci with collinearity for both SNPP and SW, of which 4 overlapping loci harbored the haplotypes with the same direction of genetic effects on SNPP and SW, indicating high possibility to simultaneously improve SNPP and SW in rapeseed. Our findings revealed both overlapping and independent loci controlling seed number per pod and seed weight in rapeseed.

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The data sets supporting the results of this article are included within the article and its additional files.

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The code and materials analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This study was funded by National Key Research and Development Program (2016YFD0100202), National Program on Key Basic Research Project of China (2015CB150201), National Nature Science Foundation of China (31471529), and the Project of Chongqing Science and Technology Commission (cstc2019jcyj-zdxmX0012, XmT2018081, and cstc2019jcyj-bshX0055).

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  1. Shuangshuang Xin and Hongli Dong contributed equally to this work

Authors and Affiliations

  1. College of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, China

    Shuangshuang Xin, Hongli Dong, Lei Yang, Dengwen Huang, Fajing Zheng, Yixin Cui, Shuang Wu, Jinghang Liao, Yajun He, Huafang Wan, Zhi Liu, Xiaorong Li & Wei Qian

  2. Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing, 400715, China

    Wei Qian

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  1. Shuangshuang Xin
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Contributions

WQ, SX, and HD conceived and designed the study. SX, HD, LY, DH, FZ, YC, SW, and JL participated in the phenotyping of seed number per pod and seed weight and performed the experiments. SX, HD, YH, HW, ZL, and XL contributed to data analysis and interpretation. SX, HD, and WQ wrote the paper. All authors reviewed the manuscript.

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Correspondence to Wei Qian.

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Xin, S., Dong, H., Yang, L. et al. Both overlapping and independent loci underlie seed number per pod and seed weight in Brassica napus by comparative quantitative trait loci analysis. Mol Breeding 41, 41 (2021). https://doi.org/10.1007/s11032-021-01232-1

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