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Circ_0072995 Promotes Ovarian Cancer Progression Through Regulating miR-122-5p/SLC1A5 Axis

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Abstract

Ovarian cancer is a common cancer affecting women with high morbidity and mortality globally. Circular RNAs (circRNAs) have been found play vital roles in multifarious cancers, including OC. This study aims to explore the biological role and underlying mechanism of circ_0072995 in OC progression. Circ_0072995 was upregulated in OC tissues and cells in a stable structure. Functional experiments indicated that circ_0072995 knockdown suppressed cell proliferation, migration, invasion and accelerated cell apoptosis of OC cells. Mechanistically, miR-122-5p was a direct target of circ_0072995, and its knockdown reversed the effects of circ_0072995 silence on inhibition of OC cell progression. Meanwhile, SLC1A5 was a downstream target gene of miR-122-5p, and miR-122-5p overexpression inhibited the progression of OC cells by targeting SLC1A5. Moreover, circ_0072995 positively regulated SLC1A5 expression via sponging miR-122-5p. Circ_0072995 could play oncogenic role in tumorigenesis and malignant development of OC by regulating miR-122-5p/SLC1A5 axis, providing a novel approach for OC treatment.

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Authors and Affiliations

  1. Key Laboratory of Birth Defects and Related Diseases of Women and Children, West China Second University Hospital, Sichuan University, Chengdu, 610066, Sichuan, China

    Xiaoqing Huang & Yongjie Luo

  2. Sichuan Cancer Hospital, No. 55, Section 4, Renmin South Road, Chengdu, 610066, Sichuan, China

    Xuejiao Li

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  1. Xiaoqing Huang
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  2. Yongjie Luo
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  3. Xuejiao Li
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Correspondence to Xuejiao Li.

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10528_2021_10092_MOESM1_ESM.tif

Supplementary Figure 1 The relative expression of miRNAs and mRNAs in OVCAR-3 and SK-OV-3 cells after circ_0072995 knockdown or miR-122-5p overexpression. (A and B) The expression levels of different miRNAs in OVCAR-3 and SK-OV-3 cells transfected with si-circ_0072995#2 or si-NC were detected by qRT-PCR. (C-E) The protein levels of different mRNAs in OVCAR-3 and SK-OV-3 cells transfected with miR-122-5p or miR-NC were tested by western blot. ***P < 0.001. Supplementary file1 (TIF 450 kb)

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Huang, X., Luo, Y. & Li, X. Circ_0072995 Promotes Ovarian Cancer Progression Through Regulating miR-122-5p/SLC1A5 Axis. Biochem Genet 60, 153–172 (2022). https://doi.org/10.1007/s10528-021-10092-5

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  • DOI: https://doi.org/10.1007/s10528-021-10092-5

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