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Effect of vernalization (Vrn) genes on root angles of bread wheat lines carrying rye translocation

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Abstract

Rye translocation is a significant genetic resource used in the development of drought-tolerant wheat varieties. The effects of vernalization genes on root angles in the reciprocal recombinant inbred population carrying rye translocation were investigated in the study. Three hundred and five bread wheat lines obtained from reciprocal recombinant inbred line population, which were found to carry rye translocation by sodium dodecyl sulfate polyacrylamide gel electrophoresis and PCR analysis, were molecularly screened for Vrn genes, and their Vrn gene combinations were determined. The seeds of the lines were germinated in petri dishes in three repetitions, and the growth angles of the roots in the early period were determined by image processing technique. The mean root angles of the lines in reciprocal populations varied between 61° and 249°. The effect of Vrn allele combinations on root angles is statistically significant. Lines carrying the dominant Vrn-D1 allele affecting the spring growth character and the gene combinations with the recessive vrn-B1 allele affecting the winter growth character generally have narrower root angles. Similarly, the dominant Vrn-A1b allele was found to be particularly effective on the narrower root angle alone. In the Tahirova-2000/Tosunbey population, the allele combination associated with the narrowest root angle was Vrn-B1 x Vrn-D1 x Vrn-A1b. The results showed that the reciprocal population carrying the 1BL.1RS rye translocation showed a phenotypic variation for root angles, and Vrn genes affected the root angles. The developed population can be used, especially in drought studies, to develop elite material carrying the rye translocation.

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Acknowledgements

This study was carried out within the scope of the project coded 02-D-18 supported by the Scientific Research Projects Commission of Karamanoğlu Mehmetbey University.

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Authors and Affiliations

  1. Plant and Animal Production Department, Vocational School of Technical Sciences, Karamanoğlu Mehmetbey University, Karaman, Turkey

    Tuğba Güleç

  2. Bioengineering Department, Engineering Faculty, Karamanoğlu Mehmetbey University, Karaman, Turkey

    Mesut Ersin Sönmez, Bedrettin Demir & Nevzat Aydın

  3. Electrical-Electronics Engineering Department, Engineering Faculty, Karamanoğlu Mehmetbey University, Karaman, Turkey

    Kadir Sabancı

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  1. Tuğba Güleç
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  2. Mesut Ersin Sönmez
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Güleç, T., Sönmez, M.E., Demir, B. et al. Effect of vernalization (Vrn) genes on root angles of bread wheat lines carrying rye translocation. CEREAL RESEARCH COMMUNICATIONS 50, 367–378 (2022). https://doi.org/10.1007/s42976-021-00188-4

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