Abstract
Microscopic examination results in patients with filariasis are often not identified by the presence of microfilaria, so it needs to be checked by Polymerase Chain Reaction (PCR). One PCR method uses High-Resolution Melting (HRM). The purpose of this study was to compare qPCR-HRM with microscopic examination methods to determine the types of microfilaria found in patients with filariasis. 19 samples were examined using a microscopic method and qPCR-HRM. The results of microscopic examination found no type of microfilaria and in qPCR-HRM identified B.malayi and W.bancrofti with peak temperature melting 78.1–78.7 ℃ and 80.2–80.8 ℃. The results of the study based on the comparison of two methods show that the types of microfilaria W.bancrofti and B.malayi can be found using qPCR-HRM.
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We would like to thank OIE Collaborating Center on Foodborne Parasites in Asian-Pacific Region for providing knowledge instruction in detail.
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The research proposal was approved by the Health Ethics Commission of the Faculty of Medicine, Padjadjaran University and informed consent was obtained from research respondents. Aspects of research use the general principles of research ethics in humans, namely: respect for human dignity, beneficence, and justice. Number of the ethical protocol: No. 0318020341.
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Ihsan, B.M., Berbudi, A., Agoes, R. et al. Microfilaria Positification Test Using Real-Time PCR Technique with HRM (High-Resolution Melting). Acta Parasit. 67, 496–503 (2022). https://doi.org/10.1007/s11686-021-00412-5
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DOI: https://doi.org/10.1007/s11686-021-00412-5