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Neonatal Proinflammatory Stress and Expression of Neuroinflammation-Associated Genes in the Rat Hippocampus

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Abstract

Differential effect of the neonatal proinflammatory stress (NPS) on the development of neuroinflammation in the hippocampus and induction of the depressive-like behavior in juvenile and adult male and female rats was studied. NPS induction by bacterial lipopolysaccharide in the neonatal period upregulated expression of the Il6 and Tnf mRNAs accompanied by the development of depressive-like behavior in the adult male rats. NPS increased expression of the mRNAs for fractalkine and its receptor in the ventral hippocampus of the juvenile male rats, but did not affect expression of mRNAs for the proinflammatory cytokines and soluble form of fractalkine. NPS downregulated expression of fractalkine mRNA in the dorsal hippocampus of juvenile males. No significant effects of NPS were found in the female rats. Therefore, the NPS induces long-term changes in the expression of neuroinflammation-associated genes in different regions of the hippocampus, which ultimately leads to the induction of neuroinflammation and development of depressive-like behavior in male rats.

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Abbreviations

DH:

dorsal hippocampus

IFN:

interferon

IL:

interleukin

LPS:

bacterial lipopolysaccharide

NPS:

neonatal proinflammatory stress

FST:

forced swimming test

P:

postnatal day

PBS:

phosphate buffered saline

SPT:

sucrose preference test

TNF:

tumor necrosis factor

VH:

ventral hippocampus

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Funding

This work was financially supported by the State Budget Program and State Task of the Ministry of Education and Science of the Russian Federation (animal behavior) and by the Russian Science Foundation (project no. 19-75-00063, morphology of microglia).

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Authors and Affiliations

  1. Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, 117485, Moscow, Russia

    Alexey A. Kvichansky, Liya V. Tret’yakova, Maria N. Volobueva, Anna O. Manolova, Mikhail Yu. Stepanichev, Mikhail V. Onufriev, Yulia V. Moiseeva, Natalia A. Lazareva, Alexey P. Bolshakov & Natalia V. Gulyaeva

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  1. Alexey A. Kvichansky
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  2. Liya V. Tret’yakova
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Correspondence to Alexey A. Kvichansky.

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Kvichansky, A.A., Tret’yakova, L.V., Volobueva, M.N. et al. Neonatal Proinflammatory Stress and Expression of Neuroinflammation-Associated Genes in the Rat Hippocampus. Biochemistry Moscow 86, 693–703 (2021). https://doi.org/10.1134/S0006297921060079

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