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Development of subgenome-specific PCR markers in the short arm of wheat and rye chromosome 1 and their utilization in wheat-rye translocation breeding

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Abstract

T1RS.1BL wheat-rye translocation has been widely used in wheat breeding owing to agronomically beneficial genes located on the short arm of rye chromosome 1 (1RS). Although numerous methods have been developed to identify wheat lines possessing 1RS, there are some limits to their practical application in large breeding populations. In this study, we developed 10 homoeologous markers specific to each short arm of chromosome 1 of common wheat and rye based on genome databases, and their specificity was validated using wheat aneuploid and rye chromosome addition lines. Furthermore, recombinant inbred lines (RILs) were developed by crossing T1RS.1BL and non-translocated wheat and screening with the developed markers. Seed storage protein analysis revealed that the RILs possessed high gluten quality, and recombination had occurred in gluten and gliadin gene coding loci, except for in the 1RS region. The root and shoot biomasses of the RILs under drought and recovery were measured, indicating superior drought tolerance of the T1RS.1BL lines. The markers and RILs developed in this study could be useful in breeding wheat-rye translocations.

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Acknowledgements

This work was carried out with the support of the “the Cooperative Research Program for Agriculture Science & Technology Development (PJ015666)” Rural Development Administration, Republic of Korea.

Funding

This work was carried out with the support of the “the Cooperative Research Program for Agriculture Science & Technology Development (PJ015666)” Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Institute of Life Science and Natural Resources, Korea University, Seoul, 02841, Korea

    Woo Joo Jung

  2. Department of Plant Biotechnology, Korea University, Seoul, 02841, Korea

    Yong Weon Seo

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  1. Woo Joo Jung
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Woo Joo Jung. The first draft of the manuscript was written by Woo Joo Jung and reviews and edited by Yong Weon Seo. All authors read and approved the final manuscript.

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Correspondence to Yong Weon Seo.

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Jung, W.J., Seo, Y.W. Development of subgenome-specific PCR markers in the short arm of wheat and rye chromosome 1 and their utilization in wheat-rye translocation breeding. Euphytica 217, 142 (2021). https://doi.org/10.1007/s10681-021-02875-z

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