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Pseudoxanthomonas beigongshangi sp. nov., a novel bacteria with predicted nitrite and nitrate reduce ability isolated from pit mud of Baijiu

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Abstract

A Gram-negative and rod-shape bacterium designated REN9T, was isolated from pit mud of Baijiu in Sichuan, China. The 16S rRNA sequence of strain REN9T had a high similarity to Pseudoxanthomonas indica P15T (99.21%), P. mexicana AMX 26BT (97.74%) and P. japonensis 12-3T (97.43%). Phylogenetic analysis showed that REN9T belongs to the genus Pseudoxanthomonas and formed distinct cluster. The ANI and DDH values between strains REN9T and P15T were 80.94% and 24%, respectively. Strain REN9T grew optimally at 37 °C, pH 7.0 and 2% NaCl. PE (phosphatidylethanolamine), PG (phosphatidylglycerol) and DPG (diphosphatidylglycerol) were the major polar lipids of REN9T. Ubiquinone 8 (Q-8) was the predominant quinone, and Iso-C15:0 (64.32%), anteiso-C15:0 (12.04%) and Iso-C14:0 (4.56%) were the majority fatty acids. The DNA G + C content of strain REN9T was 67.3 mol%. Genomic analysis showed that strain REN9T had two secondary metabolite biosynthesis gene clusters. Moreover, strain REN9T had 43 glycoside hydrolases, 41 glycosyl transferases and 41 carbohydrate esterases. Based on the polyphasic taxonomic analysis, strain REN9T is recommended as a novel species within the genus Pseudoxanthomonas, for which the name Pseudoxanthomonas beigongshangi is proposed. The type stain is REN9T (= JCM 33961T = GDMCC 1.2210T).

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Data availability

The genome and 16S rRNA gene of REN9T was submitted to GenBank and got the accession number of JADHDV000000000 and MW187782, respectively.

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Acknowledgements

This work supported by The National Key Research and Development Program of China (2018YFC1603606), Earmarked Fund for Hebei Edible Fungi Innovation Team of Modern Agro-industry Technology Research System (HBCT2018050205), and Science and Technology general project of Beijing municipal commission (KM202110011002). We are grateful to Zou Xiaohui, associate researcher at Nationl Institute For Viral Disease Control And Prevention, China CDC with preparing transmission electron microscope.

Funding

This work supported by The National Key Research and Development Program of China (2018YFC1603606), Earmarked Fund for Hebei Edible Fungi Innovation Team of Modern Agro-industry Technology Research System (HBCT2018050205), and Science and Technology general project of Beijing municipal commission (KM202110011002).

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  1. Zhanbin Sun and Fang Dai have contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Brewing Molecular Engineering of China Light Industry, Beijing Technology and Business University, Beijing, 100048, China

    Zhanbin Sun, Fang Dai, Yi Yan, Liyin Guo, Haoyue Gu, Jialiang Xu & Qing Ren

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  1. Zhanbin Sun
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  2. Fang Dai
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  3. Yi Yan
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  4. Liyin Guo
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  5. Haoyue Gu
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  7. Qing Ren
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Contributions

QR designed the experiments. ZBS, FD, LYG and HYG conducted the experiment. JLX and YY contributed to the data analysis. ZBS drafted the manuscript. QR and JLX revised the manuscript.

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Correspondence to Jialiang Xu or Qing Ren.

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Sun, Z., Dai, F., Yan, Y. et al. Pseudoxanthomonas beigongshangi sp. nov., a novel bacteria with predicted nitrite and nitrate reduce ability isolated from pit mud of Baijiu. Antonie van Leeuwenhoek 114, 1307–1314 (2021). https://doi.org/10.1007/s10482-021-01603-w

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