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Antibacterial Potential of Actinomycetes from North-Western Himalayas against Pathogenic Bacteria

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Abstract

Actinomycetes derived from unfamiliar surroundings are considered as a promising supply for novel bioactive compounds comprising a broad range of biological actions. The current study was carried out to find out the bioactive adjacent to resistant pathogenic strains. During the study, 46 strains of actinomycetes were isolated from the soil sediment sample collected from Manasbal Lake of Kashmir. During the primary screening, 14 actinomycete isolates showed an inhibition zone against MTCC pathogenic cultures, including clinical isolates of Streptomyces aureus. Out of 14 isolates, 8 showed a wide range of activity. One isolate A41 was found to be propitious for its wide spectrum of antibacterial action. Phylogenetic studies of A41 strain showed maximum resemblance with Streptomyces exfoliates MMA1033. The study was extended to standardize the fermentation conditions to enhance bioactive metabolite production. For A41, actinomycetes isolation agar (AIA) was confirmed as the best growth medium for maximum antibiotic production on the 16th day at pH 7, with an inoculum size of 2.5% (w/v) at 28°C. Peptone 1% (w/v) and sucrose 1% (w/v) were optimized as the best nitrogen and carbon sources respectively for the highest antimicrobial production. The isolates were not found to be halotolerant, exhibited the highest antibiotic production at 1.5% (w/v) of NaCl. The advance exploration could provide us extremely resourceful antibacterial agents in opposition to the clinical imperative human pathogens. To the best of our knowledge, this is the first report on an assorted assembly of Actinomycetes from freshwater lakes of Kashmir having wide spectrum antimicrobial action.

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REFERENCES

  1. Altschul, S.F., Gish, W., Miller, W., Myers, E.W., and Lipman, D.J., Basic local alignment search tool, J. Mol. Biol., 1990, vol. 215, pp. 403–410.

    Article  CAS  Google Scholar 

  2. Altschul, S.F., Thomas, L.M., Alejandro, A.S., Zhang, J., Zhang, Z., Miller, W., and Lipman, D.J., Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res., 1997, vol. 25, pp. 3389–3402.

    Article  CAS  Google Scholar 

  3. Atta, H.M., Production, purification, physico-chemical characteristics and biological activities of antifungal antibiotic produced by Streptomyces antibioticus AZ-Z710, Am.-Eurasian J. Sci. Res., 2010, vol. 5, no. 1, pp. 39–49.

    CAS  Google Scholar 

  4. Berdy, J., Bioactive microbial metabolites: a personal view, J Antibiot., 2005, vol. 58, no. 1, pp. 1–26.

    Article  CAS  Google Scholar 

  5. Bundale, S., Begde, D., Nashikkar, N., Kadam, K., and Upadhya, A., Optimization of culture conditions for production of bioactive metabolites by Streptomyces spp. isolated from soil, Adv. Microbiol., 2015, vol. 5, pp. 441–451.

    Article  Google Scholar 

  6. Das, S., Lyla, P.S., and Khan, S.A., Characterization and identification of marine actinomycetes existing systems, complexities and future directions, Natl. Acad. Sci. Lett., 2008, vol. 31, pp. 149–160.

    Google Scholar 

  7. El-Mehalawy, A.A., Abd-Allah N.A., Mohamed, R.M., and Abu-Shady, M.R., Actinomycetes antagonizing plant and human pathogenic fungi. II. Factors affecting antifungal production and chemical characterization of the active components, Int. J. Agric. Biol., 2005, vol. 7, no. 2, pp. 188–196.

    CAS  Google Scholar 

  8. Ganesan, P., Reegan, A.D., David, H.A., Gandhi, M.R., Paulraj, M.G., Al-Dhabi, N.A., and Ignacimuthu, S., Antimicrobial activity of some actinomycetes from Western Ghats of Tamil Nadu, India, Alexandria J. Med., 2017, vol. 53, pp. 101–110.

    Google Scholar 

  9. Gebreselema, G., Feleke, M., Samuel, S., and Nagappan, R., Isolation and characterization of potential antibiotic producing actinomycetes from water and sediments of Lake Tana Ethiopia, Asian Pac. J. Trop. Biomed., 2013, vol. 3, pp. 426–35.

    Article  Google Scholar 

  10. Hayakawa, M., Ishiwwa, K., and Nonomurha, H., Distribution of rare actinomycetes in Japanese soils, J. Fermt. Technol., 2004, vol. 66, pp. 367–373.

    Article  Google Scholar 

  11. Jensen, P.R., Williams, P.G., Oh, D.C., Zeigler, L., and Fenical, W., Species-specific secondary metabolite production in marine actinomycetes of the genus Salinispora, Appl. Environ. Microbiol., 2007, vol. 73, pp. 1146–1152.

    Article  CAS  Google Scholar 

  12. Kumar, P.S., Preetam, P.R., Veeramuthu, D., and Ignacimuthu, S., Antibacterial activity of some actinomycetes from Tamil Nadu. India, Asian Pac. J. Trop. Biomed., 2012, vol. 2, no. 12, pp. 936–943.

    Article  CAS  Google Scholar 

  13. Kumar, S., Stecher, G., and Tamura, K., MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets, Mol. Biol. Evol., 2016, vol. 33, pp. 1870–1874.

    Article  CAS  Google Scholar 

  14. Kumari, M., Bat-Erdene, M., Birendra, P., and Madhusudan, C., Identification and characterization of antibiotic-producing actinomycetes isolates, Am. J. Microbiol., 2013, vol. 4, no. 1, pp. 24–31.

    Google Scholar 

  15. Liu, D., Coloe, S., Baird, R., and Pedersen, J., Rapid mini-preparation of fungal DNA for PCR, J. Clin. Microbiol., 2000, vol. 8, p. 71.

    Google Scholar 

  16. Maged, S.A., Ahmed, O.E., Rasha, R.A., Hossam, M.H., Hussein, M.E., and Ahmed, G.M., Exploring the antimicrobial and antitumor potentials of Streptomyces sp. AGM12-1 isolated from Egyptian Soil, Front. Microbiol., 2017, vol. 13, p. 438.

    Google Scholar 

  17. Mudgetti, R.E., Arnolds, L.D., and Nadine, A.S., Manual of Industrial Biotechnology, Washington, DC: American Society for Microbiology, 1986.

    Google Scholar 

  18. Myn, U., Nuruddin, M., Nural, A., and Mohammed, A.M., Bioactive metabolite production by Streptomyces albolongus in favourable environment, J. Microbiol. Infect. Dis., 2013, vol. 3, no. 2, pp. 75–82.

    Article  Google Scholar 

  19. Ningthoujam, D.S., Sanasam, S., and Nimaichand, S., Screening of actinomycete isolates from niche habitats in Manipur for antibiotic activity, Am. J. Biochem. Biotechnol., 2009, vol. 5, no. 4, pp. 221–225.

    Article  Google Scholar 

  20. Oskay, M., Effects of some environmental conditions on biomass and antimicrobial metabolite production by Streptomyces sp. KGG32, Int. J. Agric. Biol., 2011, vol. 13, pp. 317–324.

    CAS  Google Scholar 

  21. Pandey, B., Ghimire, P., and Agrawal, V.P., Studies on the antimicrobial activity of the actinomycetes isolated from the Khumbu region of Nepal, Appl. Microbiol., 2008, vol. 5, pp. 235–261.

    Google Scholar 

  22. Rakesh, K.N., Junaid, S., Dileep, N., Vinayaka, K.S., Kekuda, T.R., and Raghavendra, H.L., Antibacterial and antioxidant activity of Fahrenheitia zeylanica (Thw.) Airy, Sci. Technol. Arts Res. J., 2013, vol. 2, no. 4, pp. 27–33.

    Google Scholar 

  23. Ramesh, S. and Mathivanan, N., Screening of marine actinomycetes isolated from the Bay of Bengal, India for antimicrobial activity and industrial enzymes, World J. Microbiol. Biotechnol., 2009, vol. 25, pp. 2103–2111.

    Article  CAS  Google Scholar 

  24. Reddy, N.G., Ramakrishna, D., and Rajagopal, S., Optimization of culture conditions of Streptomyces rochei (MTCC 10109) for the production of antimicrobial metabolites, Egypt. J. Biol., 2011, vol. 13, pp. 21–29.

    Google Scholar 

  25. Robinson, T., Singh, D., and Nigam, P., Solid state fermentation: a promising microbial technology for secondary metabolite production, Appl. Microbiol. Biotechol., 2001, vol. 55, pp. 284–289.

    Article  CAS  Google Scholar 

  26. Saadoun, I. and Muhana, A., Optimal production conditions, extraction, partial purification and characterization of inhibitory compound(s) produced by Streptomyces Ds-104 isolate against multi-drug resistant Candida albicans, Curr. Trends Biotechnol. Pharm., 2008, vol. 2, pp. 402–432.

    CAS  Google Scholar 

  27. Singh, V., Khan, M., Khan, S., and Tripathi, K.M., Optimization of actinomycin V production by Streptomyces triostinicus using artificial neural network and genetic algorithm, Appl. Microbiol. Biotechnol., 2009, vol. 82, pp. 379–385.

    Article  CAS  Google Scholar 

  28. Thenmozhi, M. and Kannabiran, K., Studies on isolation, classification and phylogenetic characterization of novel antifungal Streptomyces sp. VITSTK7 in India, Curr. Res. J. Biol. Sci., 2010, vol. 2, no. 5, pp. 306–312.

    Google Scholar 

  29. Thompson, J.D., Higgin, D.G., and Gibson, T.J., CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice, Nucleic Acids Res, 1994, vol. 22, pp. 4673–4680.

    Article  CAS  Google Scholar 

  30. Wadetwar, R.N. and Patil, A.T., Production of antibiotic from actinomycetes isolated from Nagpur region and optimization of parameters to increase the yield, Int. J. Pharm. Sci. Res., 2013, vol. 4, no. 8, pp. 3094–3098.

    Google Scholar 

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ACKNOWLEDGMENTS

The authors are appreciative of the Hon’ble Vice-chancellor and Management of Arni University for providing required facilities.

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Authors and Affiliations

  1. Department of Biotechnology and Microbiology, Arni School of Basic Sciences, ArniUniversity, 176401, Kathgarh Himachal Pradesh, India

    N. Salaria

  2. Department of Microbiology, SKIMS Medical College, 190018, Bemina, Srinagar, Jammu and Kashmir, India

    J. Furhan

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Correspondence to J. Furhan.

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Salaria, N., Furhan, J. Antibacterial Potential of Actinomycetes from North-Western Himalayas against Pathogenic Bacteria. Biol Bull Russ Acad Sci 48, 281–289 (2021). https://doi.org/10.1134/S1062359021030146

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