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Characterization of Pseudomonas cichorii isolated from tomato and lettuce in Iran

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Abstract

Stem and pith necrosis symptoms on tomato and midrib rot symptoms on lettuce were observed in West and East Azarbayjan of Iran. Infected organs of the plants were collected and bacterial strains were isolated on NA medium. Biochemical and physiological properties such as LOPAT test, fluorescent pigment production and utilization of carbon source, demonstrated that the strains were Pseudomonas cichorii. All strains were pathogenic on tomato and lettuce plants. To assess genetic diversity among the strains, BOX and ERIC-PCR analyses were employed. Clustering of ERIC and BOX-PCR results with UPGMA and Jaccard’s similarity coefficients showed that strains were clustered into two main groups at a similarity level of 52%. Strains of groups 1 and 2 isolated from lettuce and tomato, respectively. The gyrB, rpoD and 16S rRNA nucleotide sequences showed high similarity and the sequence similarity between the Iranian strains and P. cichorii reference strain were 98.9, 98.7 and 99.5%, indicating a close phylogenetic relationship between the strains. According to the past references and researches in Iran, this is the first report of isolation and identification of Pseudomonas cichorii, the causative agent of tomato pith necrosis and lettuce midirib rot in North West of Iran.

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Authors and Affiliations

  1. Department of Plant Protection, Islamic Azad University, Tabriz branch, Tabriz, Iran

    Shialn Arshad, Sevil Nematollahi & Kiomars Rouhrazi

  2. Department of Plant Protection, Islamic Azad University, Mahabad branch, Mahabad, Iran

    Nabi Khezrinejad

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  1. Shialn Arshad
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  2. Sevil Nematollahi
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  3. Kiomars Rouhrazi
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  4. Nabi Khezrinejad
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Correspondence to Sevil Nematollahi.

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Arshad, S., Nematollahi, S., Rouhrazi, K. et al. Characterization of Pseudomonas cichorii isolated from tomato and lettuce in Iran. J Plant Pathol 103, 853–861 (2021). https://doi.org/10.1007/s42161-021-00863-9

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  • DOI: https://doi.org/10.1007/s42161-021-00863-9

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