Abstract
As the diploid progenitor of the D genome of common wheat, Aegilops tauschii contains abundant genetic resources for potential economic traits, including yield, biotic and abiotic stress tolerance. In this study, phenotypic variation for 4 grain shapes in 221 Ae. tauschii accessions was evaluated in 5 environments for 2 consecutive crop seasons, and best linear unbiased estimates (BLUEs) for grain shapes were calculated across the seasons. To decrease false discovery rate, a total of 139 loci were only identified based on the BLUEs and each environment by genome-wide association study utilizing 4282 single nucleotide polymorphism (SNP) markers from D genome of wheat 55 K genotyping assay. These loci could explain 5.86–17.71% of the phenotypic variation of grain length (GL), 6.83–8.42% of grain width, 5.99–17.71% of grain perimeter, and 5.80–19.61% of grain length–width ratio (GLWR), respectively. Further, 26 significant loci were validated in the wheat mini-core collections population by two-tailed t test. Moreover, the physical positions of some loci for GL and GLWR were found to correspond with previously mapped loci that affect these traits. Based on the obtained SNP loci, 6 putative candidate genes related to grain shapes were identified. This study could provide valuable information for cloning genes related to grain shapes in Ae. tauschii.
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Acknowledgements
We are grateful to J. Jia (Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS)) for providing wheat mini-core collection and information. This work was supported by Project of Science and Technology Department of Henan Province (Grant No. 202102110031, 212102110269) and Key Scientific Research Projects of Higher Education Institutions in Henan Province (Grant No. 20A210007).
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Zhao, X., Lv, L., Li, J. et al. Genome-wide association study of grain shapes in Aegilops tauschii. Euphytica 217, 144 (2021). https://doi.org/10.1007/s10681-021-02877-x
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DOI: https://doi.org/10.1007/s10681-021-02877-x