Abstract
A method for the quantification of a mixture of fluorescent antibiotics with broad, highly overlapped excitation and emission spectra is proposed. The method enables the simultaneous determination of sarafloxacin, flumequine and ofloxacin by using the fluorescence excitation-emission matrix and performing two different scans, namely, a linear variable angle synchronous fluorimetry scan at 15.6° and an emission scan at λexc of 320 nm. This ensures complete resolution of the mixture without the need for a separation technique. Various factors influencing the fluorescence intensity were assessed in order to maximize the sensitivity and obtain an adequate selectivity. The method was successfully used to determine the analytes in pharmaceuticals and rabbit plasma. The results obtained compared favorably with those provided by HPLC.
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REFERENCES
Currie, D., Lynas L., Kennedy, D.G., and Mccaughey, W.J., Food Addit. Contam., 1998, vol. 15, p. 651.
Ihrke, P.J., Papich, M.G., and Demanuelle, T.C., Vet. Dermatol., 1999, vol. 10, p. 193.
Ragab Gamal, H. and Amin, A.S., Spectrochim. Acta, Part A, 2004, vol. 60, p. 973.
Golet, A.C., Hartmann Alder, A., Temes, T.A., and Giger, W., Anal. Chem., 2001, vol. 73, p. 3632.
Barrón, D., Jiménez-Lozano, E., Bailac S., and Barbosa, J., Anal. Chim. Acta, 2003, vol. 477, p. 21.
Altiokka, G., Atkosar, Z., and Can, N.O., J. Pharm. Biomed. Anal., 2002, vol. 30, p. 881.
Vílchez, J.L., Ballesteros, O., Taoufiki, J., Sánchez-Palencia, G., and Navalón, A., Anal. Chim. Acta, 2001, vol. 444, p. 279.
Ocaña González, J.A., Callejón Mochón, M., and Barragán de la Rosa, F.J., Talanta, 2000, vol. 52, p. 1149.
Arroyo-Manzanares, N., Huertas-Pérez, J.F., Lombardo-Agüï, M., Gámiz-Gracia, L., and García-Campaña, A.M., Anal. Methods, 2015, vol. 7, p. 253.
Neckel, U., Joukhadar, C., Frossard, M., Jäger, W., Müller, M., and Mayer, B.X., Anal. Chim. Acta, 2002, vol. 463, p. 199.
Pecorelli, I., Galarini, R., Bibi, R., Floridi, Al., Casciarri, E., and Floridi, A., Anal. Chim. Acta, 2003, vol. 483, p. 81.
Bailac, S., Ballesteros, O., Jiménez-Lozano, E., Barrón, D., Sanz-Nebot, V., Navalón, A., Vílchez, J.L., and Barbosa, J., J. Chromatogr. A, 2004, vol. 1029, p. 145.
Lara, F.J., del Olmo-Iruela, M., and García-Campaña, A.M., J. Chromatogr. A, 2013, vol. 1310, p. 91.
D’Agostino, P.A., Hancock, J.R., and Provost, L.R., Rapid Commun. Mass Spectrom., 1995, vol. 9, p. 1038.
Turnipseed, S.B., Roybal, J.E., Pffening, A.P., and Kijak, P.J., Anal. Chim. Acta, 2003, vol. 483, p. 373.
Ballesteros, O., Sanz-Nebot, V., Navalón, A., Vílchez, J.L., and Barbosa, J., Chromatographia, 2004, vol. 59, p. 543.
Ruiz-Viceo, J.A., Rosales-Conrado, N., Guillén-Casla, V., Pérez-Arribas, L.V., León-González, M.E., and Polo-Díez L.M., J. Food Compos. Anal., 2012, vol. 28, p. 99.
Aly, F.A., Al-Tamimi, S.A., and Alwarthan, A.A., Talanta, 2001, vol. 53, p. 885.
Rizk, M., Belal, F., Aly, F.A., and El-Enany, N.M., Talanta, 1998, vol. 46, p. 83.
Ghoneim, M.M., Radi, A., and Beltagi, A.M., J. Pharm. Biomed. Anal., 2001, vol. 25, p. 205.
Xuan, C.S., Wang, Z.Y., and Song, J.L., Anal. Lett., 1998, vol. 31, p. 1185.
Rizk, M., Belal, F., Ibrahim, F., Ahmed, S.M., and Sheribah, Z.A., J. AOAC Int., 2001, vol. 84, p. 368.
Kilic, E., Koseoglu, F., and Akayt, M.A., J. Pharm. Biomed. Anal., 1994, vol. 12, p. 347.
Andrade-Eiroa, A., de Armas, G., Estela, J.M., and Cerdá, V., TrAC, Trends Anal. Chem., 2010, vol. 29, p. 902.
John, P. and Soutar, I., Anal. Chem., 1976, vol. 48, p. 520.
Murillo Pulgarín, J.A. and Alañón Molina, A., Comput. Chem., 1993, vol. 4, p. 341.
Miller, J.N., Analyst, 1984, vol. 109, p. 191.
Murillo Pulgarín, J.A., Alañón Molina, A., and Fernández López, P., Anal. Chim. Acta, 1998, vol. 370, p. 9.
Murillo, J.A., Alañón A., Fernández, P., Muñoz de la Peña, A., and Espinosa, A., Analyst, 1998, vol. 123, p. 1073.
Murillo Pulgarín, J.A., Alañón Molina, A., and Sánchez-Ferrer Robles, I., Appl. Spectrosc., 2010, vol. 64, no. 8, p. 949.
Savitzky, A. and Golay, M.J.E., Anal. Chem., 1964, vol. 36, p. 1626.
Steinier, J., Termonia, Y., and Deltour, J., Anal. Chem., 1972, vol. 44, p. 1906.
Rousseeuw, P.J. and Leroy, A.M., Robust Regression and Outlier Detection, Oxford: Oxford Univ. Press, 1988.
Lark, P.D., Craven, B.R., and Bosworth, R.C.L, The Handling of Chemical Data, Exeter: Pergamon, 1968, ch. 4.
Massart, D.L., Vandeginste, B.G.M., Deming, S.N., and Kaufman, L., in Chemometrics: A Textbook, Vandeginste, B.G.M. and Kaufman, L., Eds., Oxford: Oxford Univ. Press, 1988.
IUPAC, Analytical Chemistry Division. Nomenclature, symbols, units and their usage in spectrochemical analysis. II, Spectrochim. Acta, Part B, 1978, vol. 33, p. 242.
Warren, D. and Crummett, B., Anal. Chem., 1980, vol. 52, p. 2242.
Miller, J.N., Analyst, 1991, vol. 116, p. 3.
Long, G.L. and Winefordner, J.D., Anal. Chem., 1983, vol. 55, p. 712A, 716A, 720A, 722A, 724A.
Clayton, C.A., Hines, J.W., and Elkins, P.D., Anal. Chem., 1987, vol. 59, p. 2506.
Cañadas-Cañadas, F., Espinosa Mansilla, A., and Muñoz de la Peña, A., J. Sep. Sci., 2007, vol. 30, p. 1242.
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The authors gratefully acknowledge financial support from the “Consejería de Educación, Cultura y Deportes de la Junta de Comunidades de Castilla-La Mancha y el Fondo Europeo de Desarrollo Regional” (PEII-2014-008-A).
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Pulgarín, J.A., Molina, A.A. & Boras, N. Use of Total Fluorescence Spectroscopy for the Highly Sensitive Simultaneous Determination of Fluoroquinolones in Rabbit Plasma. J Anal Chem 76, 733–741 (2021). https://doi.org/10.1134/S1061934821060101
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DOI: https://doi.org/10.1134/S1061934821060101